Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) antibody (Biotin)

Antigen: Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D)

Synonyms: CD100, SEMAJ, coll-4, C9orf164, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-G, MGC169138, MGC169141, Semaj, Semcl2, Semacl2

Clonality: Monoclonal (A8)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN312146

Additional Information

Alternative name: CD100

Immunogen: Human PHA activated lymphocytes

Isotype: IgG1, kappa chain

Clone: A8

Description: CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability invitro. Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development.CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulatingCD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulatingB cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NKcells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activatedT cells and B cells.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 0.5 ug per 106 cells in 100 ul volume or100 ul of whole blood. It is recommended that the reagent be titrated foroptimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated withbiotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C. Do not freeze.

Restrictions: For Research Use only