TCR Vb13.2 antibody (Biotin)

Antigen: TCR Vb13.2

Clonality: Monoclonal (H132)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN312252

Additional Information

Immunogen: Human TCR Vbeta13.2 transfectant

Isotype: IgG1, kappa chain

Clone: H132

Description: The Vbeta13.2 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset ofperipheral blood T cells and some T cell clones. Vbeta expression in humans and mice has been used to study theeffects of superantigens and also to examine the repertoires of T cells involved in inflammatory and autoimmunedisease. It is reported that Vbeta13.2 T cell receptors (TCRs) with very unusually long beta-chain complementaritydetermining region 3 (CDR3) regions in CTL specific for the immunodominant human histocompatibility leukocyteantigens (HLA)-B8&ndash,restricted human immunodeficiency virus-1 (HIV-1) nef epitope, FLKEKGGL (FL8). CTLexpressing V beta13.2 TCRs tolerate naturally arising viral variants in the FL8 epitope that escape recognition by otherCTL. The clone H132 recognizes the Vbeta13.2 allele product, but does not react with the Vbeta13.1, 13.3, 13.5 and 13.6allele products of the Vbeta13 subfamily.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume or100 ul of whole blood. It is recommended that the reagent be titrated foroptimal performance for each application. It is recommended that the reagentbe titrated for optimal performance for each application.

Purification: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody was purified by affinity chromatography, and conjugated withbiotin under optimal conditions. The solution is free of unconjugated biotin.

Restrictions: For Research Use only