Inducible T-Cell Co-Stimulator (ICOS) antibody (Biotin)

Antigen: Inducible T-Cell Co-Stimulator (ICOS)

Synonyms: AILIM, CD278, MGC39850, Ailim, ICOS, H4, Ly115

Clonality: Monoclonal (7E-17G9)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN312297

Additional Information

Alternative name: CD278 (ICOS)

Immunogen: Mouse ICOS cDNA and ICOS hexahistidine fusion protein

Isotype: IgG2b, kappa chain

Clone: 7E-17G9

Description: The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, andH4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset ofthymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved inhumoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 isconstitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS.ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed toplay a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and thedownregulation of pulmonary inflammation.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is = 0.25 ug per 106 cells in 100 ul volume. Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. Thesolution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C. Do not freeze.

Restrictions: For Research Use only

Publications

Publications: McAdam, Chang, Lumelsky et al.: "Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 5035-40, 2000 (PubMed).

Akbari, Freeman, Meyer et al.: "Antigen-specific regulatory T cells develop via the ICOS-ICOS-ligand pathway and inhibit allergen-induced airway hyperreactivity." in: Nature medicine, Vol. 8, Issue 9, pp. 1024-32, 2002 (PubMed).

Harada, Salama, Sho et al.: "The role of the ICOS-B7h T cell costimulatory pathway in transplantation immunity." in: The Journal of clinical investigation, Vol. 112, Issue 2, pp. 234-43, 2003 (PubMed).