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Antibodies
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anti-Interleukin 1 alpha (IL1A) antibody (Biotin)
Interleukin 1 alpha (IL1A) antibody (Biotin)
| Antigen |
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| Synonyms |
IL1, IL-1A, IL1F1, IL1-ALPHA, Il-1a, IL-1 alpha, Il1a |
| Clonality |
Monoclonal (BL1a-89) |
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Host
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Reactivity
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Conjugate
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Biotin (8), FITC (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
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Application
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Western Blotting (WB) (35), Enzyme Immunoassay (EIA) (24), Flow Cytometry (FACS) (21), Immunofluorescence (IF) (18), ELISA (11), Functional Studies (Func) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Blocking Antibody (Inhibition) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Neutralization (Neut) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN312335 |
| Quantity |
50 ug (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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IL-1alpha
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Immunogen
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E. coli - expressed, recombinant mouse IL-1alpha
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Isotype
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IgG2a, kappa chain
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Clone
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BL1a-89
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Description
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IL-1 refers to two proteins, IL-1alpha and IL-1beta which are the products of distinct genes, but which are recognized by thesame cell surface receptors. IL-1alpha is a potent immuno-modulator which mediates a wide range of immune andinflammatory responses. The biotinylated BL1a-89 antibody is useful as a detection antibody for a sandwich ELISA orELISPOT assay when used in conjunction with the purified BL1a-72 antibody as capture antibody and recombinantmouse IL-1alpha?(Cat. No. 562801) as the standard.
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Application Notes
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Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, aconcentration range of 0.05-0.2 ug/ml is recommended. To obtain a linear standard curve, serial dilutions of mouseIL-1alpha recobinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For Western Blotting applications, it is suggested to use 5 ug antibody per 5 ml antibody dilution buffer for each mini-gel. It isrecommended that the reagent be titrated for optimal performance for each application.
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Purification
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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. Thesolution is free of unconjugated biotin.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Storage
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The antibody solution should be stored undiluted at 4 degrees C. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "Interleukin 1 alpha (IL1A)", type "Antibodies"
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Hosts
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Rabbit (39), Mouse (14), Goat (6), Armenian Hamster (3), Sheep (1)
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Reactivities
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Human (43), Mouse (Murine) (31), Rat (Rattus) (24), Cow (Bovine) (18), Dog (Canine) (18), Pig (Porcine) (16), Sheep (Ovine) (15), Horse (Equine) (1), Monkey (1), Rabbit (1)
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Applications
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Western Blotting (WB) (35), Enzyme Immunoassay (EIA) (24), Flow Cytometry (FACS) (21), Immunofluorescence (IF) (18), ELISA (11), Functional Studies (Func) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Blocking Antibody (Inhibition) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Neutralization (Neut) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (8), FITC (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
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Alternatives 
