Ku80 antibody (Biotin)
| Antigen |
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| Clonality |
Monoclonal (F3) |
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Host
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Reactivity
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Conjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Application
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| Catalog no. |
ABIN312339 |
| Quantity |
100 ug |
| Price |
Product not available in this region.
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| Shipping to |
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Immunogen
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Recombinant
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Isotype
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IgG1, kappa chain
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Clone
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F3
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Description
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Ku80 is an 80 kD nuclear ATP-dependent DNA helicase. Ku80 is involved in dsDNA break repair, VDJrecombination, and chromosome translocation. This protein binds fork-like ends of dsDNA in cell-cycle dependentmanner. Ku80 can be modified by phosphorylation and has been shown to interact with Ku70 and DNA-PKcs. TheF3 monoclonal antibody recognizes human Ku80 and has been shown to be useful for Western blotting andimmunofluorescence staining.
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Application Notes
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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s):Use 5 ug antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use adilution range of 1-4 ug/ml. It is recommended that the reagent be titrated for optimal performance for eachapplication.
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Purification
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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. Thesolution is free of unconjugated biotin.
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Buffer
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This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
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Storage
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The antibody solution should be stored undiluted at 4 degrees C. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "Ku80", type "Antibodies"
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Hosts
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Rabbit (28), Mouse (2), Goat (1)
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Reactivities
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Human (29), Mouse (Murine) (21), Rat (Rattus) (21), Dog (Canine) (4), Horse (Equine) (4), Pig (Porcine) (4), Cow (Bovine) (3), Rabbit (2), Hamster (1)
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Applications
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Immunofluorescence (IF) (21), Flow Cytometry (FACS) (19), Western Blotting (WB) (13), ELISA (5), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (4), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr714 (1)
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Alternatives 
