TCR-Vb8.1, 8.2 antibody (Biotin)

Antigen: TCR-Vb8.1, 8.2

Clonality: Monoclonal (KJ16-133-18)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN312358

Additional Information

Alternative name: TCR Vb8.1,8.2

Immunogen: TCR receptor from T cell hybridoma DO-11.10

Isotype: IgG2a, kappa chain

Clone: KJ16-133-18

Description: The TCR Vbeta 8 family consists of three members, namely Vbeta8.1, 8.2 and 8.3. KJ16-133.18 reacts with Vbeta8.1, 8.2only, but not Vbeta8.3. Most mouse strains have about 20% Vbeta 8+ T cells in the periphery. However, C57BR, C57L,SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vbeta8 due to a gene deletion at the Vbeta 8family loci. Other strains expressing endogenous super-antigen, such as Mls-1a have significant reduced number ofVbeta 8.1 cells in the periphery. Exogenous superantigen, such as staphylococcal enterotoxin B, stimulateslymphocytes bearing Vbeta 8 and selectively eliminate those T cells in vivo.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for eachapplication.

Purification: The antibody was purified by affinity chromatography, and conjugated withbiotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C. Do not freeze.

Restrictions: For Research Use only