CD85d (ILT4) antibody

Antigen: CD85d (ILT4)

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human

Application: Flow Cytometry (FACS), Functional Studies (Func)

Catalog no.: ABIN312571

Additional Information

Isotype: IgG2a, kappa chain

Clone: 42D1

Description: CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or LeukocyteImmunoglobulin-like Receptors (LIRs). It is composed of both activating and inhibitory isoforms. The activating subsetof ILTs is characterized by containing short cytoplasmic domains and positively charged arginine residues, while theinhibitory isoforms display long cytoplasmic tails containing ITIM motifs. CD85d is a 95kD inhibitory receptor, alsoknown as ILT4, LIR2, or MIR10. ILT4 is expressed on the surface of monocytes/macrophages, and dendritic cells.ILT4 acts as an inhibitory receptor through recruitment of SHP-1 and SHP-2 protein tyrosine phosphatases. Theligands of ILT4 are HLA-A, -B and nonclassical MHC-I molecule HLA-G1.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume or100 ul of whole blood. It is recommended that the reagent be titrated foroptimal performance for each application. Additional reported applications include: negatively modulate myelomonocytic cells signaling. Enhance the binding ofHLA-G tetramer to monocytes.

Purity: LEAF

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filtersterilized. Endotoxin level is < 0.1 EU/ug of the protein (< 0.01 ng/ug of theprotein) as determined by the LAL test.

Storage: The antibody solution should be stored undiluted at 4 degrees C.

Restrictions: For Research Use only