CD276 Molecule (CD276) antibody

Antigen: CD276 Molecule (CD276)

Synonyms: B7H3, B7-H3, MGC84341, sc:d0645, zgc:158279

Clonality: Polyclonal

Host: Please enquire

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS), Functional Studies (Func)

Catalog no.: ABIN312585

Additional Information

Alternative name: CD276 (B7-H3)

Isotype: IgG2a, kappa chain

Clone: RTAA15

Description: B7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with human B7-H3. It is mostlyexpressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cellsappears to be up-regulated by LPS. B7-H3 protein inhibits T cell activation and cytokine production. It was reportedthat monoclonal antibody against B7-H3 enhanced T cell proliferation in vitro and led to exacerbated EAE in vivo.Therefore, B7-H3 may be a negative regulator on T cell activation and function.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for eachapplication.

Purity: LEAF

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filtersterilized. Endotoxin level is < 0.1 EU/ug of the protein (< 0.01 ng/ug of theprotein) as determined by the LAL test.

Storage: The antibody solution should be stored undiluted at 4 degrees C.

Restrictions: For Research Use only