CD53 (CD53) antibody

Antigen: CD53 (CD53)

Synonyms: MOX44, TSPAN25

Clonality: Monoclonal (OX-44)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN312592

Additional Information

Alternative name: CD53

Immunogen: Rat T cell blasts

Isotype: IgG1, kappa  (Matching secondary antibodies)

Clone: OX-44

Description: CD53 is a 35-45 kD type III tetraspan membrane protein. It is expressed on all leukocytes and a subset ofthymocytes, but not on platelets, erythrocytes, or non-hematopoietic cells. CD53 has been proposed to be involved insignal transduction and thymopoiesis. It has been shown to interact with a number of proteins, including IL-4, CD20,CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 promotes B cell activation. The CD53 antigen is highlyglycosylated and treatment with endoglycosidase F reduces the apparent molecular weight of this protein byapproximately 25kD.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunofluorescencemicroscopy, immunohistochemical staining for formalin-fixed paraffin-embedded tissue sections, and induction of celladhesion.

Purity: LEAF

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filtersterilized. Endotoxin level is < 0.1 EU/ug of the protein (< 0.01 ng/ug of theprotein) as determined by the LAL test.

Storage: The antibody solution should be stored undiluted at 4 degrees C.

Restrictions: For Research Use only