Integrin beta 7 (ITGB7) antibody

Antigen: Integrin beta 7 (ITGB7)

Synonyms: Ly69

Clonality: Monoclonal (FIB27)

Host: Rat

Reactivity: Mouse (Murine), Human

Application: Blocking Antibody (Inhibition), Immunoprecipitation (IP), Flow Cytometry (FACS)

Catalog no.: ABIN312703

Additional Information

Alternative name: Integrin beta7

Immunogen: TK1 cells

Isotype: IgG2a, kappa chain  (Matching secondary antibodies)

Clone: FIB27

Description: Integrin beta7 is a 130 kD glycoprotein, also known as integin betap. It is a member of the Ig superfamily. In association withintegrin alpha4 or alphaE chain, beta7 forms alpha4/beta7 or alphaE/beta7 heterodimer. alpha4/beta7 (CD49d/beta7, LPAM-1) is expressed on majority ofperipheral lymphocytes, small subsets of thymocytes and bone marrow progenitors. LPAM-1 binds to several ligands,VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion, some hematopoietic progenitor cellsmigration. alphaE/beta7 (CD103/beta7,alphaIEL/beta7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal Tcells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/beta7 complex is thought toplay a role in lymphocyte retention via interaction with its lignd E-Cadherin. The FIB27 antibody has been reported toreact with mouse and human beta7 integrin and to block beta7 integrin-mediated cell adhesion in in vitro and in vivo studies.

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking of cell adhesion1,3. The FIB27 antibody reacts with mouse and human Integrin beta7.

Purity: LEAF Purified

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filter sterilized. Endotoxin level is < 0.1 EU/ug of the protein (< 0.01 ng/ug of the protein) as determined by the LAL test.

Storage: The antibody solution should be stored undiluted at 4 degrees C.

Restrictions: For Research Use only

Publications

Publications: Andrew, Berlin, Honda et al.: "Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 9, pp. 3847-61, 1994 (PubMed).

Berlin, Berg, Briskin et al.: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, 1993 (PubMed).

Tidswell, Pachynski, Wu et al.: "Structure-function analysis of the integrin beta 7 subunit: identification of domains involved in adhesion to MAdCAM-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 3, pp. 1497-505, 1997 (PubMed).