»
Antibodies
»
anti-Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) antibody (Pa...
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) antibody (Pacific Blue)
| Antigen |
|
| Synonyms |
CD16, FCG3, CD16A, FCGR3, IGFR3, FCR-10, FCRIII, FCGRIII, FCRIIIA |
| Clonality |
Monoclonal (3G8) |
|
Host
|
|
|
Reactivity
|
 Alternatives Human, Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Marmoset
|
|
Conjugate
|
FITC (17), Biotin (14), PE (14), RPE (4), Alexa Fluor 700 (2), Cy5.5,PE (2), SPRD (2), Alexa Fluor 488 (1), PE,Cy5 (1), PE-Dyomics 647 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
|
|
Application
|
Flow Cytometry (FACS) (118), Immunoprecipitation (IP) (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Functional Studies (Func) (12), Western Blotting (WB) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunocytochemistry (ICC) (5), ELISA (3), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Blocking Antibody (Inhibition) (1), Immunohistochemistry (IHC) (1)
|
| Catalog no. |
ABIN312724 |
| Quantity |
100 tests (Variants) |
| Price |
Product not available in this region.
|
| Shipping to |
|
|
Alternative name
|
CD16
|
|
Isotype
|
IgG1, kappa chain
|
|
Clone
|
3G8
|
|
Description
|
CD16 is known as low affinity IgG receptor III (FcgammaRIII). It is expressed as two distinct (CD16a and CD16b) forms.CD16a (FcgammaRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NKcells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcgammaRIIIB) is a 48 kDglycosylphosphatidylinositol (GPI)-anchored protein. It is over 95% homologous to CD16a in extracellular domain andis expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex that functions in NK cellactivation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). The 3G8 antibody blocksneutrophil phagocytosis and stimulates NK cell proliferation.
|
|
Application Notes
|
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.
|
|
Purification
|
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue
|
|
Buffer
|
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
|
|
Storage
|
The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do notfreeze.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A)", type "Antibodies"
|
Hosts
|
Mouse (121), Rat (6), Rabbit (1)
|
|
Reactivities
|
Human (113), Pig (Porcine) (10), Primates (9), Mouse (Murine) (3), Monkey (2), Primate (1)
|
|
Applications
|
Flow Cytometry (FACS) (118), Immunoprecipitation (IP) (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Functional Studies (Func) (12), Western Blotting (WB) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunocytochemistry (ICC) (5), ELISA (3), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Blocking Antibody (Inhibition) (1), Immunohistochemistry (IHC) (1)
|
|
Conjugates
|
FITC (17), Biotin (14), PE (14), RPE (4), Alexa Fluor 700 (2), Cy5.5,PE (2), SPRD (2), Alexa Fluor 488 (1), PE,Cy5 (1), PE-Dyomics 647 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
|
