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Antibody
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anti-CD45RA CD45RA Pacific Blue
CD45RA (CD45RA) antibody (Pacific Blue)
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| Clonality |
Monoclonal (HI100) |
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Host
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Reactivity
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Conjugate
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FITC (24), Biotin (13), RPE (7), APC (5), Cy5 (5), Cy5.5 (3), Cy7 (2), Pacific Blue (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), DY647 (1), PE (1), PE-Dyomics 647 (1), PerCP (1), RPE,Cy5 (1)
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Application
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| Catalog no. |
ABIN312738 |
| Quantity |
100 tests (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD45RA
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Isotype
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IgG2b, kappa
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Clone
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HI100
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Description
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CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphataseCD45. The CD45RA isoform is expressed on resting/naive T cells, medullary thymocytes, B cells and monocytes.CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates withlymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to beassociated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reportedto bind galectin-1. CD45 isoform expression can change in response to cytokines.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.
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Purification
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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do notfreeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD45RA (CD45RA)", type "Antibodies"
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Hosts
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Mouse (145)
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Reactivities
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Human (118), Rat (Rattus) (16), Pig (Porcine) (8), Dog (Canine) (3)
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Applications
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Flow Cytometry (FACS) (124), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunoprecipitation (IP) (21), Western Blotting (WB) (17), Immunohistochemistry (IHC) (9), Immunofluorescence (IF) (7), Immunocytochemistry (ICC) (3), ELISA (1)
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Conjugates
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FITC (24), Biotin (13), RPE (7), APC (5), Cy5 (5), Cy5.5 (3), Cy7 (2), Pacific Blue (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), DY647 (1), PE (1), PE-Dyomics 647 (1), PerCP (1), RPE,Cy5 (1)
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Alternatives 
