IL-2 antibody (Pacific Blue)

Antigen: IL-2

Clonality: Monoclonal (JES6-5H4)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Pacific Blue

Application: Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN312768

Additional Information

Immunogen: E. coli-expressed, recombinant mouse IL-2

Isotype: IgG2b, kappa chain

Clone: JES6-5H4

Description: IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 hasbeen found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2). This is a neutralizing antibody.

Application Details

Application Notes: This reagent is developed for immunofluorescent staining for flow cytometricanalysis, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ulvolume. It is highly recommended that the reagent be titrated for optimalperformance for each application.* Pacific Blue

Purification: The antibody was purified by affinity chromatography, and conjugated withPacific Blue

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Images

anti-IL-2 antibody (Pacific Blue)

Publications

Publications: Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, 1995 (PubMed).

Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Karulin, Hesse, Tary-Lehmann et al.: "Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1862-72, 2000 (PubMed).

Kang, Allen: "Priming in the presence of IL-10 results in direct enhancement of CD8+ T cell primary responses and inhibition of secondary responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 9, pp. 5382-9, 2005 (PubMed).

Ko, Ko, Chang et al.: "alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 3309-17, 2005 (PubMed).

Lawson, Manenkova, Ahamed et al.: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007 (PubMed).