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Vascular Cell Adhesion Molecule 1 (VCAM1) antibody (PE)

Antigen

Vascular Cell Adhesion Molecule 1 (VCAM1)
Synonyms CD106, MGC99561, INCAM-100, DKFZp779G2333, Vcam-1, VCAM1B, MGC108734, VCAM1, fi76d06, wu:fi76d06, zgc:158875, VCAM-1, DKFZp469N1820
Clonality Monoclonal (429(MVCAM-A))
Host
Reactivity
Alternatives

Mouse (Murine)
Conjugate
Application
Alternatives Flow Cytometry (FACS)
Catalog no. ABIN312982
Quantity 50 ug  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Alternatives Variants back to top

Alternative name CD106
Immunogen Mouse preadipose cell line PA6
Isotype IgG2a, kappa chain
Clone 429(MVCAM-A)
Description CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 andINCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells,activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cellsby inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (alpha4/beta1 integrin)and LPAM-1 (alpha4/beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Application Details
Alternatives Variants back to top

Application Notes Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for eachapplication. Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 ofacetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, and immunoprecipitation1. The LEAF
Purification The antibody was purified by affinity chromatography, and conjugated withPE under optimal conditions. The solution is free of unconjugated PE andunconjugated antibody.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "Vascular Cell Adhesion Molecule 1 (VCAM1)", type "Antibodies"
Hosts Mouse (115), Rat (62), Rabbit (26), Sheep (2)
Reactivities Human (125), Mouse (Murine) (81), Rat (Rattus) (29), Monkey (5), Pig (Porcine) (5), Dog (Canine) (4), Chicken (2), Cow (Bovine) (2), Horse (Equine) (2), Rabbit (2)
Applications Flow Cytometry (FACS) (159), Immunohistochemistry (Frozen Sections) (IHC (fro)) (57), Immunoprecipitation (IP) (48), Western Blotting (WB) (38), Immunofluorescence (IF) (35), ELISA (30), Immunohistochemistry (IHC) (24), Functional Studies (Func) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Enzyme Immunoassay (EIA) (11), Blocking Antibody (Inhibition) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (30), FITC (27), PE (17), RPE (4), APC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)