CD58 (CD58) antibody (PE/Cy5)
| Antigen |
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| Synonyms |
LFA3, LFA-3, FLJ23181, FLJ43722 |
| Clonality |
Monoclonal (TS2-9) |
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Host
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Reactivity
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Conjugate
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Application
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Flow Cytometry (FACS) (69), Immunoprecipitation (IP) (27), Western Blotting (WB) (22), Functional Studies (Func) (16), Immunohistochemistry (IHC) (9), Immunofluorescence (IF) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Cytotoxicity Test (CyTox) (4), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), ELISA (2), ELISA (Detection) (2)
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| Catalog no. |
ABIN313156 |
| Quantity |
100 tests (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD58
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Immunogen
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Human cytolytic T cells
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Isotype
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IgG1, kappa chain
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Clone
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TS2-9
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Description
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CD58, also known as lymphocyte function-associated antigen 3 (LFA-3) is a 45-70 kD cell surface protein that is amember of the immunoglobulin superfamily. Alternative splicing of CD58 gives rise to transmembrane andglycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is xpressed on both hematopoietic andnon-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, andfibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on antigen presentingcells and target cells enhances T cell recognition via the binding of its cognate ligand, CD2, on the T cell surface. TheHCD58 antibody recognizes human CD58 and has been shown to be useful for flow cytometry.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 ul per million cells or 20 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application. Additional reported applications include: immunoprecipitation1, inhibition of cytolytic activity1, augment of IL-1 releaseby TE cells2
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Purification
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The antibody was purified by affinity chromatography, and conjugated withPE/Cy5 under optimal conditions. The solution is free of unconjugatedPE/Cy5 and unconjugated antibody.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD58 (CD58)", type "Antibodies"
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Hosts
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Mouse (90), Rabbit (1)
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Reactivities
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Human (80), Sheep (Ovine) (14), Pig (Porcine) (8)
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Applications
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Flow Cytometry (FACS) (69), Immunoprecipitation (IP) (27), Western Blotting (WB) (22), Functional Studies (Func) (16), Immunohistochemistry (IHC) (9), Immunofluorescence (IF) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Cytotoxicity Test (CyTox) (4), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), ELISA (2), ELISA (Detection) (2)
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Conjugates
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Biotin (13), FITC (13), PE (7), RPE (4), APC (2), Alexa Fluor 488 (1), Cy5,PE (1), Pacific Blue (1)
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Alternatives 
