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CD45.1 antibody (PE/Cy7)

Antigen

CD45.1
Clonality Monoclonal (A20)
Host
Alternatives

Mouse
Reactivity
Alternatives

Mouse (Murine)
Conjugate
Alternatives PE/Cy7
Application
Alternatives Flow Cytometry (FACS)
Catalog no. ABIN313189
Quantity 25 ug  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Alternatives Variants back to top

Immunogen SJL mouse thymocytes and splenocytes
Isotype IgG2a, kappa
Clone A20
Description CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, amember of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on allhematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play keyroles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states ofthe cell, as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, andThy-1. The A20 antibody does not react with leukocytes that express the CD45.2 alloantigen.

Application Details
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Application Notes Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vitro blocking of B cellresponses1,2, and immunohistochemical staining3 of acetone-fixed frozen sections. The A20 antibody does not reactwith mouse cells expressing the CD45.2 alloantigen.
Purification The antibody was purified by affinity chromatography, and conjugated withPE/Cy7 under optimal conditions. The solution is free of unconjugatedPE/Cy7 and unconjugated antibody.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "CD45.1", type "Antibodies"
Hosts Mouse (34), Rat (5)
Reactivities Mouse (Murine) (34), Rat (Rattus) (5)
Applications Flow Cytometry (FACS) (39), Immunoprecipitation (IP) (12), Immunofluorescence (IF) (3)
Conjugates Cy5 (7), FITC (6), Cy5.5 (5), Biotin (4), APC (3), Cy7 (3)