HLA-ABC (HLA-ABC) antibody (PerCP)

Antigen: HLA-ABC (HLA-ABC)

Synonyms: HLAA, FLJ26655

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human, Rhesus Monkey, Cynomolgus, Baboon

Conjugate: PerCP

Application: Flow Cytometry (FACS)

Catalog no.: ABIN313227

Additional Information

Alternative name: HLA-A, B, C

Isotype: IgG2a, kappa chain

Clone: W6-32

Description: The W6/32 monoclonal antibody reacts with a non-polymorphic epitope of human major histocompatibility complex(MHC) class I antigens, HLA-A, B, C. MHC class I antigens associated with beta2-microglobulin are expressed by allhuman nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play animportant role in cell-mediated immune responses and tumor surveillance. W6/32 cross reacts with rhesus,cynomogus, baboon, feline, and bovine.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul ofwhole blood. It is recommended that the reagent be titrated for optimalperformance for each application.* PerCP has a maximum absorption of 482 nm and 564 nm and a maximumemission of 675 nm. Additional reported applications (for the relevant formats) include:immunoprecipitaton2, Western blotting (non-reducing)3,Immunohistochemical staining4,5 of acetone-fixed frozen tissue sections,blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. The LEAF

Purification: The antibody was purified by affinity chromatography, and conjugated withPerCP under optimal conditions. The solution is free of unconjugated PerCPand unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only