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Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (PerCP)

Antigen

Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)
Synonyms LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA, PTPRC, si:dkeyp-47c5.1
Clonality Monoclonal (RA3-6B2)
Host
Reactivity
Alternatives

Mouse (Murine), Human
Conjugate
Alternatives PerCP
Application
Alternatives Flow Cytometry (FACS)
Catalog no. ABIN313256
Quantity 100 ug  (Variants)
Price Product not available in this region.
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anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (PerCP)

Additional Information
Application Details Images Alternatives Variants back to top

Alternative name CD45R/B220
Immunogen Abelson murine leukemia virus-induced pre-B tumor cells
Isotype IgG2a, kappa chain
Clone RA3-6B2
Description CD45R is an isoform of CD45, also known as B220. It is a member of the protein tyrosine phosphatase (PTP) familywith a molecular weight approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages frompro-B cells through mature B cells), activated B cells, subsets of T and NK cells. CD45R (B220) is also expressed ona subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgldmice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, andCD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cellspecificity.

Application Details
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Application Notes Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and 564 nm and a maximum emission of 675 nm. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivomodulation of B cell responses2-4, and immunohistochemistry5,6 of acetone-fixed frozen sections and formalin-fixedparaffin-embedded sections. The LEAF
Purification The antibody was purified by affinity chromatography, and conjugated withPerCP under optimal conditions. The solution is free of unconjugated PerCPand unconjugated antibody.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Alternatives
Application Details Images Variants back to top

Alternatives for antigen "Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)", type "Antibodies"
Hosts Mouse (331), Rat (207), Rabbit (18)
Reactivities Human (248), Mouse (Murine) (186), Rat (Rattus) (60), Dog (Canine) (32), Chicken (27), Cow (Bovine) (18), Sheep (Ovine) (15), Pig (Porcine) (10), Cat (Feline) (6), Bird (Avian) (5), Goat (3), Primate (3), Guinea Pig (2), Rabbit (2), Monkey (1)
Applications Flow Cytometry (FACS) (481), Immunohistochemistry (Frozen Sections) (IHC (fro)) (137), Immunoprecipitation (IP) (109), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (83), Western Blotting (WB) (47), Immunohistochemistry (IHC) (24), Immunofluorescence (IF) (19), ELISA (16), Immunocytochemistry (ICC) (9), Enzyme Immunoassay (EIA) (8), Cytotoxicity Test (CyTox) (4), Functional Studies (Func) (2), Radioimmunoassay (RIA) (2), To be determined by user (TBD) (1)
Conjugates FITC (86), PE (55), Biotin (54), APC (28), RPE (14), SPRD (8), Cy5 (6), PE,Cy5 (5), Cy5,PE (4), Cy5.5,PE (4), PE,Cy7 (4), Alexa Fluor 488 (3), Cy7,PE (3), PE,Cy5.5 (3), PerCP (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy5.5 (2), PE,DY647 (2), Pacific Blue (2), RPE,Cy5 (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), PE,DY590 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1)
Epitopes C-Term (3)