I-A/I-E antibody (PerCP)

Antigen: I-A/I-E

Clonality: Monoclonal (M5-114-15-2)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: PerCP

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN313275

Additional Information

Immunogen: Activated C57BL/6 mouse spleen cells

Isotype: IgG2b, kappa chain

Clone: M5-114-15-2

Description: The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHCclass II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and asubset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCRand CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2p,r haplotypes. The M5/114.15.2 antibody hasbeen reported to block antigen presentation.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining,the suggested use of this reagent is approx. 0.25 ug per 106cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and 564 nm and a maximum emission of 675 nm. Additional reported applications (for the relevant formats) include: immunoprecipitation1, intracellularimmunofluorescent staining2, immunohistochemistry2,3,6 of frozen sections, and in vitro and in vivo blocking4-7 ofantigen presentation or ligand binding. The LEAF

Purification: The antibody was purified by affinity chromatography, and conjugated withPerCP under optimal conditions. The solution is free of unconjugated PerCPand unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Publications

Publications: Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, 1982 (PubMed).

Guo, Watanabe, Mori et al.: "Molecular structure and function of CD4 on murine egg plasma membrane." in: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, 1995 (PubMed).

Viville, Neefjes, Lotteau et al.: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, 1993 (PubMed).

Yamashita, Nagata, Tada et al.: "CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection." in: International immunology, Vol. 5, Issue 9, pp. 1139-50, 1994 (PubMed).

Nelson, Hosier, Brady et al.: "Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2453-61, 1993 (PubMed).

Shi, Kaliyaperumal, Lu et al.: "Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain." in: The Journal of experimental medicine, Vol. 187, Issue 3, pp. 367-78, 1998 (PubMed).

Kim, Noh, Kim et al.: "Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination." in: Experimental & molecular medicine, Vol. 36, Issue 5, pp. 428-43, 2004 (PubMed).

Luckashenak, Ryszkiewicz, Ramsey et al.: "The Src homology 2 domain-containing leukocyte protein of 76-kDa adaptor links integrin ligation with p44/42 MAPK phosphorylation and podosome distribution in murine dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5177-85, 2006 (PubMed).

Venanzi, Gray, Benoist et al.: "Lymphotoxin pathway and Aire influences on thymic medullary epithelial cells are unconnected." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5693-700, 2007 (PubMed).