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Antibodies
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anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (PerCP)
Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (PerCP)
| Antigen |
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| Synonyms |
TAP, Sca1, Sca-1, Ly-6A.2, Ly-6A/E, Ly-6E.1, Ly6a |
| Clonality |
Monoclonal (D7) |
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Host
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Reactivity
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Conjugate
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PE (10), Biotin (7), APC (4), FITC (4), RPE (2), PE,Cy5 (1), PE,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy5.5 (1), SPRD (1)
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Application
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| Catalog no. |
ABIN313277 |
| Quantity |
25 ug (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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Ly-6A/E (Sca-1)
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Immunogen
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IL-2-dependent mouse T-cell line (CTL-L)
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Isotype
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IgG2a, kappa chain
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Clone
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D7
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Description
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Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is aglycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing theLy-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral Blymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA,C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression ofLy-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thoughtto be involved in the regulation of both T and B cell responses. The D7 antibody has been reported to induce T cellactivation and inhibit TCR-induced IL-2 production.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and 564 nm and a maximum emission of 675 nm. Additional reported applications (for the relevant formats) include: Western blot analysis1,2, immunoprecipitation1, invitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, andimmunohistochemical staining of acetone-fixed frozen sections9.
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Purification
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The antibody was purified by affinity chromatography, and conjugated withPerCP under optimal conditions. The solution is free of unconjugated PerCPand unconjugated antibody.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "Lymphocyte Antigen 6 Complex, Locus A (Ly6a)", type "Antibodies"
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Hosts
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Rat (42), Mouse (5), Rabbit (1)
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Reactivities
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Mouse (Murine) (48), Rat (Rattus) (4)
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Applications
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Flow Cytometry (FACS) (46), Western Blotting (WB) (14), Immunoprecipitation (IP) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Functional Studies (Func) (2), Blocking Antibody (Inhibition) (1), Cytotoxicity Test (CyTox) (1)
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Conjugates
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PE (10), Biotin (7), APC (4), FITC (4), RPE (2), PE,Cy5 (1), PE,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy5.5 (1), SPRD (1)
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Alternatives 
