Mouse anti-Human IgM Antibody (PerCP/Cy5.5)
| Antigen |
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| Clonality |
Monoclonal (MHM-88) |
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Host
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Reactivity
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Human (173), Mouse (Murine) (88), Rat (Rattus) (34), Chicken (27), Rabbit (14), Cow (Bovine) (7), Monkey (7), Dog (Canine) (6), Pig (Porcine) (6), Bird (Avian) (5), Goat (5), Cat (Feline) (3), Guinea Pig (3), Horse (Equine) (3), Sheep (Ovine) (3)
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Conjugate
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FITC (64), HRP (57), Biotin (42), Alkaline Phosphatase (AP) (31), PE (22), TRITC (18), Texas Red (TR) (13), APC (9), DyLight 350 (3), DyLight 405 (3), DyLight 488 (3), DyLight 550 (3), DyLight 594 (3), DyLight 633 (3), DyLight 650 (3), DyLight 680 (3), DyLight 800 (3), Cy5 (2), PE,Cy7 (2), RPE (2), Rhodamine (2), SPRD (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Poly-HRP (1), beta-Gal (1)
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Application
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Flow Cytometry (FACS)
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| Catalog no. |
ABIN313287 |
| Quantity |
25 tests (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Immunogen
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human IgGs and IgM, IgE, IgA cocktail
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Isotype
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IgG1, kappa chain
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Clone
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MHM-88
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Description
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The MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react withother Ig isotypes. IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressedon immature and mature B cells, while IgM heavy (u) chain is expressed intracellularly in pre-B cells. The MHM-88antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is 5 ul per 106 cells in 100 ul. It isrecommended that the reagent be titrated for optimal performance for eachapplication. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and amaximum emission of 690 nm.
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Purification
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The antibody was purified by affinity chromatography, and conjugated withPerCP/Cy5.5 under optimal conditions. The solution is free of unconjugatedPerCP/Cy5.5 and unconjugated antibody.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "IgM", type "Secondary Antibodies"
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Hosts
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Goat (234), Mouse (70), Rabbit (51), Rat (15), Sheep (7), Donkey (2)
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Reactivities
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Human (173), Mouse (Murine) (88), Rat (Rattus) (34), Chicken (27), Rabbit (14), Cow (Bovine) (7), Monkey (7), Dog (Canine) (6), Pig (Porcine) (6), Bird (Avian) (5), Goat (5), Cat (Feline) (3), Guinea Pig (3), Horse (Equine) (3), Sheep (Ovine) (3)
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Conjugates
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FITC (64), HRP (57), Biotin (42), Alkaline Phosphatase (AP) (31), PE (22), TRITC (18), Texas Red (TR) (13), APC (9), DyLight 350 (3), DyLight 405 (3), DyLight 488 (3), DyLight 550 (3), DyLight 594 (3), DyLight 633 (3), DyLight 650 (3), DyLight 680 (3), DyLight 800 (3), Cy5 (2), PE,Cy7 (2), RPE (2), Rhodamine (2), SPRD (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Poly-HRP (1), beta-Gal (1)
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Epitopes
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Fc specific (25), Fc-Region, Chain mu (3)
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Alternatives 
