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Antibodies
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anti-CD33 Molecule (CD33) antibody (PerCP/Cy5.5)
CD33 Molecule (CD33) antibody (PerCP/Cy5.5)
| Antigen |
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| Synonyms |
CD33, p67, SIGLEC3, FLJ00391, SIGLEC-3 |
| Clonality |
Monoclonal (WM53) |
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Host
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Reactivity
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Conjugate
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PE (17), FITC (14), Biotin (10), APC (9), Cy5,PE (6), RPE (4), Cy5.5 (2), Cy7,PE (2), Alexa Fluor 700 (1), CF (1), Cy5 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,DY647 (1), PE-Dyomics 647 (1), PerCP (1), RPE,Cy5 (1), SPRD (1)
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Application
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Flow Cytometry (FACS) (108), Western Blotting (WB) (23), Immunoprecipitation (IP) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (5), ELISA (3), Enzyme Immunoassay (EIA) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunofluorescence (IF) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN313354 |
| Quantity |
25 tests (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD33
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Isotype
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IgG1, kappa chain
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Clone
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WM53
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Description
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CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesionimmunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells andmast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm. Additional reported applications (for the relevant formats) include: immunoprecipitation, Westrn blotting3, induction ofcytokine production3, and immunohistochemical staining of acetone-fixed frozen tissue sections. The LEAF
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Purification
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The antibody was purified by affinity chromatography, and conjugated withPerCP/Cy5.5 under optimal conditions. The solution is free of unconjugatedPerCP/Cy5.5 and unconjugated antibody.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD33 Molecule (CD33)", type "Antibodies"
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Hosts
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Mouse (116), Rabbit (5)
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Reactivities
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Human (120), Primates (7), Primate (2)
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Applications
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Flow Cytometry (FACS) (108), Western Blotting (WB) (23), Immunoprecipitation (IP) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (5), ELISA (3), Enzyme Immunoassay (EIA) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunofluorescence (IF) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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PE (17), FITC (14), Biotin (10), APC (9), Cy5,PE (6), RPE (4), Cy5.5 (2), Cy7,PE (2), Alexa Fluor 700 (1), CF (1), Cy5 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,DY647 (1), PE-Dyomics 647 (1), PerCP (1), RPE,Cy5 (1), SPRD (1)
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Epitopes
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N-Term (2)
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Alternatives 
