Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) antibody (PerCP/Cy5.5)

Antigen: Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA)

Synonyms: HFSP, CTLA3, Hf, SE1, TSP1, Ctla3, TSP-1, Ctla-3, AW494114, gzmA, GZMA, MGC157236, MET1, LMET1

Clonality: Monoclonal (CB9)

Host: Mouse

Reactivity: Human

Conjugate: PerCP,Cy5.5

Application: Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN313395

Additional Information

Alternative name: Granzyme A

Immunogen: Purified human Granzyme A

Isotype: IgG1, kappa chain

Clone: CB9

Description: Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTLgranules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophilcathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. TheCB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry,immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm. Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixedparaffin-embedded tissue sections, and immunoprecipitation2.

Purification: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions.The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do notfreeze.

Restrictions: For Research Use only