Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1) antibody (PerCP/Cy5.5)

Antigen: Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1)

Synonyms: SLAM, CD150, CDw150, SLAMF1, RGD1560634

Clonality: Monoclonal (TC15-12F12-2)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: PerCP,Cy5.5

Application: Flow Cytometry (FACS)

Catalog no.: ABIN313425

Additional Information

Alternative name: CD150 (SLAM)

Immunogen: Mouse SLAM-human IgG1 fusion protein

Isotype: IgG2a, kappa chain

Clone: TC15-12F12-2

Description: CD150 is a 75-95 kD member of the immunoglobulin superfamily also known as SLAM (signaling lymphocyteactivation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, Tcell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150expression has been shown to be maintained on Th1, but not Th2 clones. T regulatory cells express a relatively highlevel of CD150. Antibodies against CD150 have been shown to augment IFN-gamma production by Th1 cells, especiallywhen co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosinephosphatase SHP-2, and this association is thought to be involved in signal transduction. The TC15-12F12.2 antibodyhas been reported to enhance the production of IFN-gamma by Th1 cells stimulated through TCR.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm. Additional reported applications (for the relevant formats) include: immunoprecipitaion1, enhancing1 IFN-gamma productionby Th1 cells when stimulated with CD3, and inhibiting 6 CD3 induced T cell proliferation. The LEAF

Purification: The antibody was purified by affinity chromatography, and conjugated withPerCP/Cy5.5 under optimal conditions. The solution is free of unconjugatedPerCP/Cy5.5 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only