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Antibodies
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anti-CD24 Molecule (CD24) antibody (PerCP/Cy5.5)
CD24 Molecule (CD24) antibody (PerCP/Cy5.5)
| Antigen |
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| Synonyms |
HSA, Cd24, Ly-52, nectadrin, CD24A, FLJ22950, FLJ43543, MGC75043 |
| Clonality |
Monoclonal (M1-69) |
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Host
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Reactivity
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Conjugate
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FITC (27), PE (21), Biotin (14), Cy5,PE (4), APC (3), Cy5.5 (3), RPE (3), SPRD (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), RBITC (1)
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Application
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Flow Cytometry (FACS) (162), Immunohistochemistry (Frozen Sections) (IHC (fro)) (46), Immunofluorescence (IF) (24), Western Blotting (WB) (22), Immunoprecipitation (IP) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (IHC) (8), ELISA (4), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN313439 |
| Quantity |
25 ug (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD24
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Immunogen
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C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
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Isotype
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IgG2b, kappa chain
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Clone
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M1-69
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Description
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CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linkedsialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, anddendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineatingvarious lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T andB lymphocyte activation and differentiation by homophilic binding or binding to CD62P.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm. Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocytematuration2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections,zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections. The LEAF
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Purification
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The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions.The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do notfreeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD24 Molecule (CD24)", type "Antibodies"
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Hosts
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Mouse (77), Rat (72), Rabbit (24)
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Reactivities
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Human (98), Mouse (Murine) (92), Rat (Rattus) (21), Cow (Bovine) (1), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Applications
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Flow Cytometry (FACS) (162), Immunohistochemistry (Frozen Sections) (IHC (fro)) (46), Immunofluorescence (IF) (24), Western Blotting (WB) (22), Immunoprecipitation (IP) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (IHC) (8), ELISA (4), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (27), PE (21), Biotin (14), Cy5,PE (4), APC (3), Cy5.5 (3), RPE (3), SPRD (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), RBITC (1)
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Alternatives 
