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CD80 (CD80) antibody (PerCP/Cy5.5)

Antigen

CD80 (CD80)
Synonyms LAB7, CD28LG, CD28LG1
Clonality Monoclonal (16-10A1)
Host
Alternatives

Hamster
Reactivity
Alternatives

Mouse (Murine)
Conjugate
Alternatives PerCP,Cy5.5
Application
Alternatives Flow Cytometry (FACS)
Catalog no. ABIN313479
Quantity 25 ug  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Alternatives Variants back to top

Alternative name CD80
Immunogen CHO cell line transfected with mouse B7 (CD80)
Isotype IgG
Clone 16-10A1
Description CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily, also known as B7-1, B7 and Ly-53.CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed onactivated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (suchas dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation,proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The 16-10A1 antibody hasbeen shown to block the co-stimulation of T cells by activated B cells in vitro and to block the binding of CTLA-4 toCD80.

Application Details
Alternatives Variants back to top

Application Notes Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for eachapplication. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and amaximum emission of 690 nm. Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking2-4 of CTLA-4 Ig to CD80, and immunohistochemical staining1,4 of acetone-fixed frozen sections. The LEAF
Purification The antibody was purified by affinity chromatography, and conjugated withPerCP/Cy5.5 under optimal conditions. The solution is free of unconjugatedPerCP/Cy5.5 and unconjugated antibody.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "CD80 (CD80)", type "Antibodies"
Hosts Mouse (111), Rat (61), Rabbit (53), Hamster (18), Goat (2)
Reactivities Human (115), Mouse (Murine) (110), Rat (Rattus) (29), Chicken (6), Dog (Canine) (4), Rabbit (3), Baboon (2), Cow (Bovine) (2), Horse (Equine) (2), Pig (Porcine) (1)
Applications Flow Cytometry (FACS) (188), Western Blotting (WB) (53), Immunofluorescence (IF) (49), Immunoprecipitation (IP) (48), ELISA (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Functional Studies (Func) (16), Immunohistochemistry (IHC) (12), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Neutralization (Neut) (1)
Conjugates FITC (27), PE (27), Biotin (26), APC (8), Cy5,PE (6), PE,Cy5 (4), Alexa Fluor 488 (3), RPE (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
Epitopes AA 45-63 (1), extra-cellular domain (1)