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IL-10 antibody
| Antigen | IL-10 |
| Clonality | Monoclonal (JES3-12G8) |
| Host |
 Alternatives Rat |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Immunohistochemistry (IHC)
|
 |
5 references available |
| Catalog no. | ABIN313698 |
| Quantity | 50 ug (Variants) |
| Price | Product not available in this region. |
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Additional Information
| Immunogen | COS-expressed recombinant human IL-10 |
| Format | Purified |
| Isotype | IgG2a, kappa chain (Matching secondary antibodies) |
| Clone | JES3-12G8 |
| Description | IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokineproduction by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. Thebiological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayedtype hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibodyproduction. The JES3-12G8 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-12G8 antibody canneutralize the bioactivity of natural or recombinant IL-10. |
Application Details
| Application Notes | For ELISA detection applications, a concentration range of 0.25-1.0 ug/ml is recommended. It is recommended thatthe reagent be titrated for optimal performance for each application. ELISA or ELISPOT1-4: JES3-12G8 antibody is useful as a detection antibody in a sandwich ELISA or ELISPOTassay, when used in conjunction with the purified JES3-3-9D7 antibody (Cat. No. 501402/501407) or purifiedJES3-19F1 antibody (Cat. No. 506802/506810) as capture antibody and recombinant human IL-10 (Cat. No. 561201)as the standard. Flow Cytometry: The fluorochrome-labeled JES3-12G8 antibody is useful for intracellular immunofluorescent stainingand flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. Neutralization1,2: The LEAF |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4 degrees C. |
| Restrictions | For Research Use only |
Publications
| Publications |
Yssel, De Waal Malefyt, Roncarolo et al.: "IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2378-84, 1992 (PubMed).
Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed). Gotlieb, Abrams, Watson et al.: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." in: Cytokine, Vol. 4, Issue 5, pp. 385-90, 1992 (PubMed). Andersson, Abrams, Bjoerk et al.: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995 (PubMed). Burdin, Pueronne, Banchereau et al.: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." in: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, 1993 (PubMed). |
Alternatives
Alternatives for antigen "IL-10", type "Antibodies"
| Hosts | Rabbit (45), Rat (11), Mouse (6) |
| Reactivities | Human (45), Mouse (Murine) (45), Rat (Rattus) (37), Dog (Canine) (29), Cow (Bovine) (28), Pig (Porcine) (26), Rabbit (23), Horse (Equine) (11), Chicken (7), Sheep (Ovine) (3), Guinea Pig (1) |
| Applications | Flow Cytometry (FACS) (36), Immunofluorescence (IF) (35), ELISA (29), Western Blotting (WB) (18), Immunoprecipitation (IP) (6), Blocking Antibody (Inhibition) (4), Immunohistochemistry (IHC) (4), Intracellular Staining (IS) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Blocking Antibody (Inhibition) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Transfected cell culture (TCC) (2) |
| Conjugates | Biotin (7), FITC (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Flour 488 (1), RPE (1) |
