IL-32alphabetadelta antibody

Antigen: IL-32alphabetadelta

Clonality: Monoclonal (KU32-56)

Host: Mouse

Reactivity: Human

Application: ELISA (Capture), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN313713

Additional Information

Immunogen: Recombinant human IL-32alpha

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: KU32-56

Description: Interleukin 32 (IL-32), previously known as a transcript (NK4), is produced by mitogen-activated lymphocytes, by IFNgamma-activated epithelial cells or by IL-12 and IL-18-activated NK cells. Its expression is increased following activation ofT-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-kappaB and p38 MAPK cytokine signalpathways. It has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such asrheumatoid arthritis. IL-32 is unusual in that it does not share sequence homology with known cytokine families and ishighly expressed in immune tissues. IL-32 exists in at least four differentially spliced isoforms (alpha, beta, gamma and delta) withpredicted molecular weight: ~26 kDa. IL-32alpha is the shortest and most abundant of four potential splice variants of thepro-inflammatory cytokine IL-32. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32alpha was more likely to be cell-associated as compared to IL-32beta, suggesting an intracellular function.

Application Details

Application Notes: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, aconcentration range of 4-6 ug/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-32recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended thatthe reagent be titrated for optimal performance for each application. ELISA1 Capture: The purified KU32-56 antibody is useful as a capture antibody in a sandwich ELISA assay, whenused in conjunction with biotinylated KU32-52 antibody as the detection antibody for measuring human IL-32. Additional reported applications (for the relevant formats) include: Western blotting, immunofluorescence andimmunoprecipitation.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C.

Restrictions: For Research Use only