Aurora Kinase A (AURKA) (pSer66) antibody

Antigen: Aurora Kinase A (AURKA)

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Epitope: pSer66

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF)

Catalog no.: ABIN313783

Additional Information

Alternative name: Aurora A (Aurora 2)-Phosphorylated (Ser66)

Immunogen: Modified peptide

Format: Purified

Isotype: IgG  (Matching secondary antibodies)

Clone: Poly6323

Description: Aurora A (also known as Aurora 2) is a serine/threonine kinase with a molecular weight of approximately 46 kD. Thiskinase is highly expressed in the thymus and some tumors and is also expressed in other tissues including the lung,testis, colon, placenta, and fetal liver. Aurora A localizes in the midzone or central spindle in late anaphase and isconcentrated in the midbody in telophase and during cytokinesis. This kinase is believed to act in cell cycle regulationduring anaphase and/or telophase at centrosome/spindle pole during chromosome segregation. Aurora A has beenshown to regulate cleavage of polar spindle microtubules at the onset of cytokinesis during mitosis. Defects in AuroraA cause numerous centrosome aberrations including aneuploidy (genetic variant with amino acid substitution F31I).Aurora A expression is cell cycle regulated, low in G1/S, and accumulating in G2/M. Expression is upregulated incancer cells during M phase. Phosphorylation by PKA has been shown to regulate function. Aurora A phosphorylationhas been reported on Ser66. This kinase associates with the centrosome and mitotic spindles, NM23-H1, proteinphosphatase type I, and co-localizes with gamma-tubulin. The Phe 31 variant has been shown to interact with the E2ubiquitin-conjugating enzyme, UBE2N. The Poly6323 antibody has been shown to react with phosphorylated humanAurora A.

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescencemicroscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimalperformance for each application.

Purification: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Storage: Upon receipt, store frozen at -20 degrees C.

Restrictions: For Research Use only