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anti-Aurora A (Aurora 2)-Phosphorylated (Thr288) antibody

Overview

Antigen: Aurora A (Aurora 2)-Phosphorylated (Thr288)
Clonality: Monoclonal (AIK137)
Application: Western Blotting (WB)
Reactivity: Human
Host: Mouse
Quantity: 25 ug (Product variants)
Price: Product not available in this region.
Order number: ABIN313785
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Additional Information  » anti-Aurora A (Aurora 2)-Phosphorylated (Thr288) antibody

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Antigen Aurora A (Aurora 2)-Phosphorylated (Thr288)
Immunogen Modified peptide-KLH
Reactivity Human
Clonality Monoclonal
Format Purified
Isotype Mouse IgG1, kappa »Matching secondary antibodies
Description Aurora A (also known as Aurora 2) is a serine/threonine kinase with amolecular weight of approximately 46 kD. This kinase is highly expressed inthe thymus and some tumors and is also expressed in other tissues includingthe lung, testis, colon, placenta, and fetal liver. Aurora A localizes in themidzone or central spindle in late anaphase and is concentrated in themidbody in telophase and during cytokinesis. This kinase is believed to act incell cycle regulation during anaphase and/or telophase at centrosome/spindlepole during chromosome segregation. Aurora A has been shown to regulatecleavage of polar spindle microtubules at the onset of cytokinesis duringmitosis. Defects in Aurora A cause numerous centrosome aberrationsincluding aneuploidy (genetic variant with amino acid substitution F31I).Aurora A expression is cell cycle regulated, low in G1/S, and accumulating inG2/M. Expression is upregulated in cancer cells during M phase.Phosphorylation by PKA has been shown to regulate function. Aurora Aphosphorylation has been reported on Thr 288. This kinase associates withthe centrosome and mitotic spindles, NM23-H1, protein phosphatase type I,and co-localizes with gamma-tubulin. The Phe 31 variant has been shown tointeract with the E2 ubiquitin-conjugating enzyme, UBE2N. The AIK137mouse monoclonal antibody has been shown to react with phosphorylatedhuman Aurora A.
Clone AIK137
Host Mouse

Application Details  » anti-Aurora A (Aurora 2)-Phosphorylated (Thr288) antibody

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Application Western Blotting (WB)
» Click here to view 2 available protocols!
Application Notes Each lot of this antibody is quality control tested by Western blotting. ForWestern blotting, the suggested antibody concentration is 1-4 ug/ml. It isstrongly recommended that immunoprecipitation with a pan-specific Auroraantibody be carried out before Western blotting with the phospho-specificantibody to increase signal.
Purification The antibody was purified by antigen-affinity chromatography.
Buffer This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide at 0.5 mg/ml.
Storage Upon receipt, store undiluted at 4 degrees C.
Restrictions For Research Use only