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Antibody
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anti-BCL2-Associated X Protein BAX
BCL2-Associated X Protein (BAX) antibody
| Antigen |
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| Synonyms |
BCL2L4, bax, fj16e01, wu:fc50b10, wu:fj16e01, BAX, bax-A, xBax, xlbax, MGC76049, BAX-ALPHA |
| Clonality |
Monoclonal (2D2) |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (IHC)
Western Blotting (WB) (90), Immunofluorescence (IF) (59), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (40), Immunoprecipitation (IP) (36), Flow Cytometry (FACS) (26), ELISA (18), Immunohistochemistry (IHC) (13), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (EM) (2)
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| Catalog no. |
ABIN313787 |
| Quantity |
25 ug (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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Bax
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Immunogen
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Amino acid: 3-16 of human Bax protein
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Format
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Purified
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Isotype
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IgG1, kappa (Matching secondary antibodies)
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Clone
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2D2
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Description
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Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, andnucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds theanti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins suchas Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptoticstimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome cto pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionallyactivate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. Clone 2D2 has been shown to be useful for western blotting, immunoprecipitation, immunofluorescence andimmunohistochemistry of the human Bax protein. This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.
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Application Notes
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Each lot of this antibody is quality control tested by Western blotting. ForWestern blotting, suggested working dilution(s): Use 5 ug per 5 ml antibodydilution buffer for each mini-gel. It is recommended that the reagent betitrated for optimal performance for each application.
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Purification
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This antibody was purified by affinity chromatography.
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Buffer
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This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide at 0.5 mg/ml.
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Storage
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Upon receipt, store undiluted at 4 degrees C.
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Restrictions
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For Research Use only
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Alternatives for antigen "BCL2-Associated X Protein (BAX)", type "Antibodies"
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Hosts
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Mouse (79), Rabbit (57), Sheep (3)
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Reactivities
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Human (122), Mouse (Murine) (67), Rat (Rattus) (61), Dog (Canine) (31), Pig (Porcine) (31), Cow (Bovine) (30), Sheep (Ovine) (30), Gerbil (2), Monkey (1)
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Applications
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Western Blotting (WB) (90), Immunofluorescence (IF) (59), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (40), Immunoprecipitation (IP) (36), Flow Cytometry (FACS) (26), ELISA (18), Immunohistochemistry (IHC) (13), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (EM) (2)
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Conjugates
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Biotin (8), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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N-Term (2), universal (1)
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