Add to Basket
Order hotline:
 |
+1 404 474 4654 |
 |
+1 888 205 9894 (TF) |
B-Cell CLL/lymphoma 2 (BCL2) antibody
| Antigen | B-Cell CLL/lymphoma 2 (BCL2) |
| Synonyms | Bcl-2, bcl-2, BCL2 |
| Clonality | Monoclonal (BCL-10C4) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC)
|
| Catalog no. | ABIN313795 |
| Quantity | 25 ug (Variants) |
| Price | Product not available in this region. |
| Shipping to |
|
Additional Information
| Alternative name | Bcl-2 |
| Immunogen | N-terminal, Amino acid residues 61-76 of mouse Bcl-2 |
| Format | Purified |
| Isotype | IgG1, kappa (Matching secondary antibodies) |
| Clone | BCL-10C4 |
| Description | Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Tworeported isoforms exist alpha=25 kD, beta=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 familymembers. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, andendoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability.Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cellcycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinasephosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3,and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination.Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence ofthe mouse and rat Bcl-2 protein. |
Application Details
| Application Notes | Each lot of this antibody is quality control tested by Western blotting. ForWestern blotting, suggested working dilution(s): Use 5 ug per 5 ml antibodydilution buffer for each mini-gel. For immunofluorescence microscopy: Use adilution range of 1~4 ug/ml. It is recommended that the reagent be titrated foroptimal performance for each application. |
| Purification | This antibody was purified by affinity chromatography. |
| Buffer | This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide at 0.5 mg/ml. |
| Storage | Upon receipt, store undiluted at 4 degrees C. |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
| Hosts | Rabbit (103), Mouse (28), Goat (4), Sheep (3) |
| Reactivities | Human (126), Mouse (Murine) (51), Rat (Rattus) (50), Dog (Canine) (36), Cow (Bovine) (30), Pig (Porcine) (30), Rabbit (17), Chicken (15), Horse (Equine) (15), Cat (Feline) (2), Gerbil (1) |
| Applications | Western Blotting (WB) (85), Immunofluorescence (IF) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), Immunoprecipitation (IP) (38), Flow Cytometry (FACS) (33), Immunohistochemistry (IHC) (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), ELISA (19), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (EM) (2) |
| Conjugates | PE (3), Alexa Flour 488 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2) |
| Epitopes | pSer70 (7), pThr56 (7), BH3 Domain Specific (1) |
