Transgene Insertion 2, Craig B Thompson (Bcl-xS) antibody

Antigen: Transgene Insertion 2, Craig B Thompson (Bcl-xS)

Synonyms: Bcl-xS

Clonality: Monoclonal (2H12)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Pig (Porcine)

Application: Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (IHC)

Catalog no.: ABIN313799

Additional Information

Alternative name: Bcl-XS/L

Immunogen: Amino acids: 3-14 common to human and mouse Bcl-X

Format: Purified

Isotype: IgG2a, kappa  (Matching secondary antibodies)

Clone: 2H12

Description: Bcl-X is one of the members of Bcl-2 family proteins, and exists in several isoforms generated by alternative splicingand plays a critical role in the control of apoptosis. The large isoform Bcl-XL (30KD) contains the highly conservedBH1 and BH2 amino acid domains and protects cells against apoptosis, whereas the short isoform, Bcl-XS (18KD)which deletes the conserved BH1/BH2 domain by alternative mRNA splicing, promotes apoptosis by interacting withBcl-XL and Bcl-2. Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developinglymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. Clone2H12 has been shown to be useful for western blotting and immunoprecipitation, immunohistochemistry, andimmunofluorescence of the Human, Mouse and Rat Bcl-XL and Bcl-XS proteins.

Application Details

Application Notes: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested workingdilution(s): Use 5 ug per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titratedfor optimal performance for each application.

Purification: This antibody was purified by affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide at 0.5 mg/ml.

Storage: Upon receipt, store undiluted at 4 degrees C.

Restrictions: For Research Use only