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Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody

Antigen

Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9)
Synonyms MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c, Mch6, AI115399, AW493809, Caspase-9, CASP9, LOC100101592
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN313819
Quantity 50 ul  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name Caspase 9
Immunogen peptide mapping to an internal domain within the p10 subunit of humancaspase-9
Format Purified
Clone Poly6342
Description Caspase-9 is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as aheterotetramer and has been reported to have two isoforms. Pro-Caspase-9 has been reported to be approximately47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase-9 isinvolved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase-3 andCaspase-6. Caspase-9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. Thiscaspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1,granzyme B, Caspase-3, possibly Caspase-8 and Caspase-10 into large p37 and small p10 subunits. Caspase-9interacts with BIRC7 and has been shown to cleave PARP and vimentin. The Poly6342 antibody has been shown tobe useful for western blotting of human Human, Mouse and rat caspase-9 (p10) and caspase-9 precursor.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 10 ul per 5 ml antibodydilution buffer for each mini-gel. It is recommended that the reagent betitrated for optimal performance for each application.
Purification This antibody was purified by affinity chromatography.
Buffer This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide and 0.2% gelatin.
Storage The antibody solution should be stored undiluted at 4 degrees C. DO NOT FREEZE.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9)", type "Antibodies"
Hosts Rabbit (106), Mouse (13), Goat (1)
Reactivities Human (109), Rat (Rattus) (41), Mouse (Murine) (39), Dog (Canine) (21), Cow (Bovine) (19), Gerbil (3), Hamster (3), Monkey (3), Pig (Porcine) (3), Rabbit (3), Sheep (Ovine) (1)
Applications Western Blotting (WB) (89), Immunofluorescence (IF) (43), Flow Cytometry (FACS) (27), ELISA (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunoprecipitation (IP) (16), Immunohistochemistry (IHC) (15), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (EM) (3)
Conjugates Alexa Flour 488 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
Epitopes Center (4), AA 299-318 (3), active (3), pSer196 (3), AA 41-56 (2), C-Term (2), Ab-125 (1), Ab-144 (1), Ab-153 (1), Ab-196 (1), Cleaved-Asp315 (1), Cleaved-Asp330 (1), Cleaved-Asp353 (1), IN1 (1), IN2 (1), Intermediate Domain 1 (1), Intermediate Domain 2 (1), N-Term (1), S196 (1), active/cleaved (1), pS196 (1), pSer144 (1), pThr125 (1), pTyr153 (1)