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Antibodies
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anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody
| Antigen |
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| Synonyms |
MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c, Mch6, AI115399, AW493809, Caspase-9, CASP9, LOC100101592 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (87), Immunofluorescence (IF) (54), Flow Cytometry (FACS) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), ELISA (22), Immunohistochemistry (IHC) (15), Immunoprecipitation (IP) (15), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN313820 |
| Quantity |
200 ul (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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Caspase 9
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Immunogen
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peptide mapping to an internal domain within the p10 subunit of humancaspase-9
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Format
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Purified
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Clone
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Poly6342
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Description
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Caspase-9 is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as aheterotetramer and has been reported to have two isoforms. Pro-Caspase-9 has been reported to be approximately47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase-9 isinvolved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase-3 andCaspase-6. Caspase-9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. Thiscaspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1,granzyme B, Caspase-3, possibly Caspase-8 and Caspase-10 into large p37 and small p10 subunits. Caspase-9interacts with BIRC7 and has been shown to cleave PARP and vimentin. The Poly6342 antibody has been shown tobe useful for western blotting of human Human, Mouse and rat caspase-9 (p10) and caspase-9 precursor.
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Application Notes
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Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 10 ul per 5 ml antibodydilution buffer for each mini-gel. It is recommended that the reagent betitrated for optimal performance for each application.
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Purification
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This antibody was purified by affinity chromatography.
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Buffer
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This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide and 0.2% gelatin.
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Storage
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The antibody solution should be stored undiluted at 4 degrees C. DO NOT FREEZE.
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Restrictions
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For Research Use only
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Alternatives for antigen "Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9)", type "Antibodies"
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Hosts
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Rabbit (113), Mouse (10), Goat (1)
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Reactivities
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Human (114), Rat (Rattus) (44), Mouse (Murine) (42), Dog (Canine) (20), Cow (Bovine) (19), Monkey (3), Hamster (2), Pig (Porcine) (2), Rabbit (2), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (87), Immunofluorescence (IF) (54), Flow Cytometry (FACS) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), ELISA (22), Immunohistochemistry (IHC) (15), Immunoprecipitation (IP) (15), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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active (3), pSer196 (3), AA 299-318 (2), C-Term (2), Center (2), AA 41-56 (1), Ab-125 (1), Ab-144 (1), Ab-153 (1), Ab-196 (1), Cleaved-Asp315 (1), Cleaved-Asp330 (1), Cleaved-Asp353 (1), Intermediate Domain 2 (1), N-Term (1), S196 (1), pS196 (1), pSer144 (1), pThr125 (1), pTyr153 (1)
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Alternatives 
