CD53 (CD53) antibody
| Antigen |
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| Synonyms |
MOX44, TSPAN25 |
| Clonality |
Monoclonal (OX-44) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF), Induction of Cell Adhesion (ICA)
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| Catalog no. |
ABIN313901 |
| Quantity |
500 ug (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD53
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Immunogen
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Rat T cell blasts
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Format
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Purified
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Isotype
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IgG1, kappa chain (Matching secondary antibodies)
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Clone
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OX-44
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Description
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CD53 is a 35-45 kD type III tetraspan membrane protein. It is expressed on all leukocytes and a subset ofthymocytes, but not on platelets, erythrocytes, or non-hematopoietic cells. CD53 has been proposed to be involved insignal transduction and thymopoiesis. It has been shown to interact with a number of proteins, including IL-4, CD20,CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 promotes B cell activation. The CD53 antigen is highlyglycosylated and treatment with endoglycosidase F reduces the apparent molecular weight of this protein byapproximately 25kD.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for eachapplication. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunofluorescencemicroscopy, immunohistochemical staining for formalin-fixed paraffin-embedded tissue sections, and induction of celladhesion.
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Purification
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The antibody was purified by affinity chromatography.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Storage
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The antibody solution should be stored undiluted at 4 degrees C.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD53 (CD53)", type "Antibodies"
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Hosts
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Mouse (50), Rat (4), Rabbit (1)
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Reactivities
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Human (42), Rat (Rattus) (9), Mouse (Murine) (3)
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Applications
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Flow Cytometry (FACS) (51), Immunohistochemistry (Frozen Sections) (IHC (fro)) (23), Immunoprecipitation (IP) (22), Western Blotting (WB) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Functional Studies (Func) (7), Immunohistochemistry (IHC) (5), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunofluorescence (IF) (1)
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Conjugates
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FITC (11), PE (5), Biotin (2), Alexa Fluor 488 (1), Cy5,PE (1), RPE (1)
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