DNA-Damage-Inducible Transcript 3 (DDIT3) antibody

Antigen: DNA-Damage-Inducible Transcript 3 (DDIT3)

Synonyms: CHOP, CEBPZ, CHOP10, GADD153, MGC4154, chop, CHOP-10, gadd153, Chop10, Gadd153, MGC124604, Xchop, cebpz, chop10, DDIT3, MGC152049

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN313931

Additional Information

Alternative name: CHOP (GADD153)

Immunogen: KLH-peptide (RAGKQRMKEKEQENER)

Format: Purified

Clone: Poly6436

Description: CHOP (also known as growth arrest and DNA-damage-inducible protein GADD153) is a 27 kD member of the C/EBPtranscription factor family containing a basic region involved in DNA binding and a leucine zipper domain. Thisnuclear protein functions as a dimer to inhibit the DNA-binding activity of C/EBP and LAP by forming heterodimersthat cannot bind DNA target sequences. CHOP (GADD153) is induced by endoplasmic-reticulum stress and nutrientdeprivation. Phosphorylation by p38-MAP kinase enhances activity of this protein while CK2 phosphorylationdecreases activity. CHOP (GADD153) has been shown to interact with c-jun, c-fos, C/EBP, and LAP. The Poly6436antibody has been shown to be useful for Western blotting of the human CHOP protein.

Application Details

Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s):Use 10 ul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimalperformance for each application.

Purification: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Storage: Upon receipt, store frozen at -20 degrees C.

Restrictions: For Research Use only