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Histone H3 (pSer28) antibody

Antigen

Histone H3
Synonyms
H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, M ... show more
Epitope
Alternatives

pSer28
Clonality Monoclonal (HTA28)
Host
Reactivity
Alternatives

Human
Application
Alternatives Western Blotting (WB), Immunofluorescence (IF)
Catalog no. ABIN313989
Quantity 25 ug  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name Histone H3-Phospho (S28)
Immunogen Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 ofHuman Histone H3.
Format Purified
Isotype IgG2a, kappa chain  (Matching secondary antibodies)
Clone HTA28
Description H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore,limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication andchromosomal stability. During mitosis, H3 is phosphorylated at serine 28. This phosphorylation coincides withchromosome condensation initiated at prophase and disappears at late anaphase. H3 has been demonstrated to bephosphorylated by the action of MLTK-alpha (mixed linage kinase-like mitogen activated protein triple kinase alpha) inresponse to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.
Synonyms H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, FLJ35099, FLJ56780, FLJ56787, MGC131639, MGC89877, HIST1H3A

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Each lot of this antibody is quality control tested by Western blotting. Westernblotting, suggested working dilution(s): Use 5 ug antibody per 5 ml antibodydilution buffer for each mini-gel. It is recommended that the reagent betitrated for optimal performance for each application.
Purification The antibody was purified by protein G affinity chromatography
Buffer This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Storage Upon receipt, store at 4 degrees C.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Histone H3", type "Antibodies"
Hosts Rabbit (137), Mouse (15), Sheep (4)
Reactivities Human (145), Rat (Rattus) (74), Mouse (Murine) (73), Cow (Bovine) (23), Pig (Porcine) (19), Rabbit (19), Fruit Fly (Drosophila melanogaster) (17), Dog (Canine) (7), Chicken (4), Horse (Equine) (3), Sheep (Ovine) (3), Guinea Pig (1), Tetrahymena (1)
Applications Western Blotting (WB) (96), Immunofluorescence (IF) (75), Flow Cytometry (FACS) (49), ELISA (43), Enzyme Immunoassay (EIA) (29), Dot Blot (Dot) (23), Immunohistochemistry (IHC) (21), Chromatin Immunoprecipitation (ChiP) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 488 (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), PE,Cy5.5 (7), Agarose Beads (1), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer10 (23), pSer28 (17), pThr3 (12), pThr11 (6), 3meLys79 (2), Mono Methyl Lysine 4 (2), N-Term (2), Tri Methyl Lysine 4 (2), 2meArg17 (1), 2meLys27 (1), 2meLys27, 3meLys27 (1), 2meLys36 (1), 2meLys4 (1), 2meLys79 (1), 3meLys27 (1), 3meLys27, pSer10 (1), 3meLys4 (1), 3meLys9 (1), 3meLys9, pSer10 (1), Ab-23 (1), Ab-3 (1), Acetyl-Lys14 (1), Acetyl-Lys18 (1), Acetyl-Lys23 (1), Acetyl-Lys27 (1), Acetylated (1), Arg2 (1), acLys9 (1), acLys9, pSer10 (1), acetyl-Lys9 (1), meLys27 (1), meLys36 (1), meLys4 (1), meLys79 (1), meLys9 (1)