X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5)

Clonality: Monoclonal (235-1)

Host: Mouse

Reactivity: Human

Application: Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN314020

Additional Information

Alternative name: Ku (Ku-70/Ku-80)

Immunogen: CEM human T-cell leukemia cells

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: 235-1

Description: Ku, is a heterodimeric DNA end binding complex composed of 70 KD and 80 kD subunits, serves as the DNA bindingcomponent of the DNA-dependent protein kinase (DNA-PK). Ku binds to double-stranded DNA breaks (DSBs),activates the catalytic subunit (DNA-PKcs) when DNA is bound, and is essential in DSB repair and V(D)Jrecombination. Clone 235-1 has been reported to bind the holoprotein (70KD and 80KD heterodimer) and both chainsare need for the antigen to be recognized by this antibody. Clone 235-1 has been shown to be useful forImmunoprecipitation and Immunofluorescence staining. This clone is not suitable for Western blotting.

Application Details

Application Notes: Each lot of this antibody is quality control tested by Immunofluorescence staining. Forimmunofluorescence microscopy, suggested working dilution(s): Use a dilution range of 2-4 ug/ml. Forimmunoprecipitation, this antibody can be used at 2-4 ug /1x107 cell equivalents. It is recommended that the reagentbe titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.

Storage: The antibody solution should be stored undiluted at 4 degrees C.

Restrictions: For Research Use only