Linker For Activation of T Cells (LAT) antibody

Antigen: Linker For Activation of T Cells (LAT)

Synonyms: LAT1, pp36, MGC108666, LAT, MGC157208, lat

Clonality: Monoclonal (11B-12)

Host: Mouse

Reactivity: Mouse (Murine), Human

Application: Western Blotting (WB)

Catalog no.: ABIN314022

Additional Information

Alternative name: LAT

Immunogen: GST fusion protein corresponding to residues 31-233 of human LAT,cytoplasmic domain.Reactivity: HumanCross-

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: 11B-12

Description: LAT is a transmembrane molecule that mediates signaling following the engagement of a number of cell surfacereceptors including TCR during mature T cell activation, pre-TCR during T cell development, FcgRIII on NK cells, andFcepsilonRI on mast cells. LAT is multiply phosphorylated on tyrosines by ZAP-70 and Syk, which leads to downstreameffects that include release of intracellular calcium, PKC activation, MAPK activation and cytoskeletal reorganization. Palmitoylation of LAT is required for its localization to lipid rafts and efficient phosphorylation.

Application Details

Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s):Use 5 ug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titratedfor optimal performance for each application.

Purification: The antibody was purified by protein G affinity chromatography

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.

Storage: Upon receipt, store at 4 degrees C

Restrictions: For Research Use only