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Antibodies
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anti-Nanog Homeobox Pseudogene 8 (NANOGP8) antibody
Nanog Homeobox Pseudogene 8 (NANOGP8) antibody
| Antigen |
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| Synonyms |
ENK, ecat4, 2410002E02Rik, PN8, NANOG, NANOGP1, MGC119250, nanog |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (110), Mouse (Murine) (31), Rat (Rattus) (24), Dog (Canine) (5), Chicken (3), Cow (Bovine) (3), Rabbit (2), Zebrafish (Brachydanio rerio) (2), Goat (1), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Application
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Western Blotting (WB) (80), Immunofluorescence (IF) (51), ELISA (37), Flow Cytometry (FACS) (37), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
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| Catalog no. |
ABIN314057 |
| Quantity |
50 ul (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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Nanog
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Immunogen
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Peptide-KLH
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Format
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Purified
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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Poly6320
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Description
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Nanog (Homeo box transcription factor nanog) is a transcription factor,express in embryonic stem (ES) and carcinoma (EC) cells. Nanog bindsoptimally to the DNA consensus sequence5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cellsto enter into S phase and proliferation. Nanog involved in inner cell mass andembryonic stem (ES) cells proliferation and self-renewal. Imposespluripotency on ES cells and prevents their differentiation towardsextraembryonic endoderm and trophectoderm lineages. The poly6320antibody recognizes human Nanog and has been shown to be useful forWestern Blotting.
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Application Notes
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Each lot of this antibody is quality control tested by Western blotting. Westernblotting, suggested working dilution(s): Use at 1:250-1:500 dilutions. It isrecommended that the reagent be titrated for optimal performance for eachapplication.
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Purification
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The antibody was purified by antigen-affinity chromatography.
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Buffer
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This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide and 50% glycerol.
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Storage
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Upon receipt, store frozen at -20 degrees C.
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Restrictions
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For Research Use only
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Alternatives for antigen "Nanog Homeobox Pseudogene 8 (NANOGP8)", type "Antibodies"
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Hosts
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Rabbit (84), Mouse (24), Goat (8)
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Reactivities
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Human (110), Mouse (Murine) (31), Rat (Rattus) (24), Dog (Canine) (5), Chicken (3), Cow (Bovine) (3), Rabbit (2), Zebrafish (Brachydanio rerio) (2), Goat (1), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (80), Immunofluorescence (IF) (51), ELISA (37), Flow Cytometry (FACS) (37), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
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Conjugates
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Biotin (7), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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C-Term (3), Center (3), N-Term (2), S285 (2), S71 (1)
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