Phospho-ERalpha (Ser167) antibody

Antigen: Phospho-ERalpha

Epitope: Ser167

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF)

Catalog no.: ABIN314079

Additional Information

Alternative name: Phospho-ERalpha (Ser167)

Immunogen: Modified peptide

Format: Purified

Isotype: IgG  (Matching secondary antibodies)

Clone: Poly6365

Description: ERalpha (Estrogen receptor alpha) belongs to the nuclear hormone receptorfamily, NR3 subfamily. Contains an N-terminal domain with ahormone-independent transcriptional activation function (AF-1), a centralDNA-binding domain, and a C-terminal ligand-binding domain with ahormone-dependent transcriptional activation function (AF-2). Predictedmolecular weight approximately 66 kD. Estrogen receptor has two subtypes,ERalpha and ERbeta. Liganded ERalpha and ERbeta binds as a homodimer or as ahetrodimer with an estrogen responsive element and regulate the target genetranscription. Estrogen receptors mediate most of the biological effects ofestrogen in mammary and uterine epithelial cells. High expression of ERs isobserved in a large population of breast tumors. It has been reported AKT1,AKT2, p90RSK3, or p90RSK1 are capable to phosphorylate ser-167 of thehuman ER and Ser-167 may play a role in controlling AF-1 function. ThePoly6365 antibody recognizes human phospho-ERalpha (Ser 167) and has beenshown to be useful for immunoflorence staining. This antibody does notdetect ERalpha phosphorylated at other sites.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescencestaining. For immunofluorescence microscopy: Use a dilution range of1:100~1:400. It is recommended that the reagent be titrated.

Purification: This antibody was purified by affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide and 50% glycerol.

Storage: Upon receipt, store frozen at -20 degrees C.

Restrictions: For Research Use only