RARalpha (0) (pSer62) antibody

Antigen: RARalpha (0)

Epitope: pSer62

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF)

Catalog no.: ABIN314089

Additional Information

Alternative name: Phospho-RXRalpha (Ser62)

Immunogen: Modified peptide

Format: Purified

Isotype: IgG  (Matching secondary antibodies)

Clone: Poly6329

Description: RXRalpha (Retinoic acid receptor RXR alpha) is a 51KD member of the nuclear hormone receptor family, NR2 subfamily.This nuclear protein contains three domains: a modulating N-terminal domain, a DNA-binding domain and aC-terminal steroid-binding domain. The expression of RXRalpha is modulated differently during the activation of cellcycling. There are three retinoic acid receptors (RXR), RXRalpha, RXR, and RXRgamma encoded by the RXRA, RXRB, RXRGgenes respectively. RXRs play an important role as promiscuous heterodimerization partners in modulating theactivities of a number of nuclear receptors, which include RAR, thyroid hormone receptor, vitamin D receptor,peroxisome proliferator activated receptors, and COUP-TF. The poly6329 antibody recognizes human phosphorylatedRXRalpha(Ser61) and has been shown to be useful for immunoflorence staining.

Application Details

Application Notes: Each lot of this antibody is quality control tested by Immunofluorescencestaining. For immunofluorescence microscopy, suggested working dilution(s):Use a dilution range of 1:00~1:400. For IHC, use a 1:50 dilution of antibodyfor staining. Antigen retrieval for IHC of formalin-fixed paraffin-embeddedtissue using 0.01 M sodium citrate buffer is recommended. It isrecommended that the reagent be titrated for optimal performance for eachapplication.

Purification: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide and 50% glycerol.

Storage: Upon receipt, store frozen at -20 degrees C.

Restrictions: For Research Use only