SIK2 antibody

Antigen: SIK2

Clonality: Monoclonal (S15G10)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN314109

Additional Information

Immunogen: Recombinant human SIK2

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: S15G10

Description: SIK2 (Salt inducible kinase 2) belongs to the protein kinase superfamily,CAMK Ser/Thr protein kinase family, and AMP-activated protein kinase(AMPK) subfamily, predicted molecular weight 104 kD. SIK2 is found inadipocytes and might be involved in metabolic regulation in adipose tissue.Sik2 phosphorylates ser794 of human insulin receptor substrate-1 (IRS1) ininsulin-stimulated adipocytes, playing important roles in modulating theinsulin-signaling cascade of adipocytes. Clone S15G10 has been shown tobe useful for western blotting, immunoprecipitation, and immunoflorencestaining of human SIK2 protein.

Application Details

Application Notes: Each lot of this antibody is quality control tested by Western blotting. ForWestern blotting, suggested working dilution(s): Use 5 ug per 5 ml antibodydilution buffer for each mini-gel. For immunofluorescence microscopy: Use adilution range of 1~4 ug/ml. It is recommended that the reagent be titrated foroptimal performance for each application. Description: SIK2 (Salt inducible kinase 2) belongs to the protein kinase superfamily,CAMK Ser/Thr protein kinase family, and AMP-activated protein kinase(AMPK) subfamily, predicted molecular weight 104 kD. SIK2 is found inadipocytes and might be involved in metabolic regulation in adipose tissue.Sik2 phosphorylates ser794 of human insulin receptor substrate-1 (IRS1) ininsulin-stimulated adipocytes, playing important roles in modulating theinsulin-signaling cascade of adipocytes. Clone S15G10 has been shown tobe useful for western blotting, immunoprecipitation, and immunoflorencestaining of human SIK2 protein.

Purification: This antibody was purified by affinity chromatography.

Buffer: This antibody is prodived in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide at 0.5 mg/ml.

Storage: Upon receipt, store undiluted at 4 degrees C.

Restrictions: For Research Use only