Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 300-400) antibody (Biotin)

Details for Product No. ABIN314157
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Antigen
Synonyms EGLN1, egln2, phd2, zgc:92314, AI503754, C1orf12, HIF-PH2, HPH-2, Hif-p4h-2, ORF13, Phd2, SM-20, PHD-2, PHD2, ECYT3, HIFPH2, HPH2, SM20, ZMYND6
Epitope
AA 300-400
(15), (11), (11), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(73), (27), (12), (12), (6), (1), (1), (1)
Host
Rabbit
(53), (28), (1)
Clonality
Polyclonal
Conjugate
Biotin
(6), (5), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(64), (18), (16), (13), (10), (10), (9), (7), (5), (3)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN314157
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Immunogen A synthetic peptide made to an internal portion of mouse PHD2/HIF Prolyl Hydroxylase 2 (between residues 300-400).
Cross-Reactivity (Details) Predicted to react with rat,based on 100% sequence homology.
Purification affinity purified
Alternative Name EGLN1 / PHD2
Background HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen.HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. Ittargets HIF through the hydroxylation for proteasomal degradation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-PHD2 antibody, anti-C1ORF12 antibody, anti-Chromosome 1 Open Reading Frame12 antibody, anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1antibody, anti-HIF Prolyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2antibody, anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody,anti-PHD2 antibody, anti-EGLN1 antibody.
Gene Symbol: EGLN1
Gene ID 54583
UniProt Q91YE3
Application Notes This HIF Prolyl Hydroxylase 2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin embedded sections and Immunoprecipitation. In Western blot a band is seen ~43 kDa representing HIF Prolyl Hydroxylase 2. There is also a non-specific band of similar intensity at ~75 kDa.
Recommended dilutions: immunohistochemistry 2.5µg/mL, immunoprecipitation 1:10-1:500, Western Blot 2 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
General Mikhaylova, Ignacak, Barankiewicz et al.: "The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2701-17, 2008 (PubMed).

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