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Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 300-400) antibody (HRP)

Details for Product No. ABIN314158, Supplier: Log in to see
Antigen
  • EGLN1
  • egln2
  • phd2
  • zgc:92314
  • AI503754
  • C1orf12
  • HIF-PH2
  • HPH-2
  • Hif-p4h-2
  • ORF13
  • Phd2
  • SM-20
  • PHD-2
  • PHD2
  • ECYT3
  • HIFPH2
  • HPH2
  • SM20
  • ZMYND6
Epitope
AA 300-400
18
13
13
8
5
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
81
27
13
2
1
1
Host
Rabbit
61
19
2
Clonality
Polyclonal
Conjugate
HRP
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
58
34
23
15
11
5
2
2
2
1
1
1
1
Supplier
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Immunogen A synthetic peptide made to an internal portion of mouse PHD2/HIF Prolyl Hydroxylase 2 (between residues 300-400).
Cross-Reactivity (Details) Predicted to react with rat,based on 100% sequence homology.
Purification affinity purified
Alternative Name EGLN1 / PHD2 (EGLN1 Antibody Abstract)
Background HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen.HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. Ittargets HIF through the hydroxylation for proteasomal degradation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-PHD2 antibody, anti-C1ORF12 antibody, anti-Chromosome 1 Open Reading Frame12 antibody, anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1antibody, anti-HIF Prolyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2antibody, anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody,anti-PHD2 antibody, anti-EGLN1 antibody.
Gene Symbol: EGLN1
Gene ID 54583
UniProt Q91YE3
Pathways
Application Notes This HIF Prolyl Hydroxylase 2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin embedded sections and Immunoprecipitation. In Western blot a band is seen ~43 kDa representing HIF Prolyl Hydroxylase 2. There is also a non-specific band of similar intensity at ~75 kDa.
Recommended dilutions: immunohistochemistry 2.5µg/mL, immunoprecipitation 1:10-1:500, Western Blot 2 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
Product cited in: Mikhaylova, Ignacak, Barankiewicz et al.: "The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2701-17, 2008 (PubMed).