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RPE65 antibody (Retinal Pigment Epithelium-Specific Protein 65kDa) (Biotin)

Details for Product anti-RPE65 Antibody No. ABIN314161, Supplier: Log in to see
Antigen
  • 65kDa
  • A930029L06Rik
  • LCA2
  • Mord1
  • mRPE65
  • rd12
  • RP20
  • RPE56c
  • si:ch211-198n5.12
  • sRPE65
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
46
46
39
30
21
19
18
18
1
1
1
Host
Mouse
26
24
9
Clonality (Clone)
Monoclonal ()
Conjugate
This RPE65 antibody is conjugated to Biotin
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
46
24
20
18
17
16
14
12
11
5
2
1
Supplier
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Immunogen Bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]
Clone 401-8B11-3D9
Isotype IgG1 kappa
Specificity This antibody is specific for RPE65.
Cross-Reactivity (Details) This antibody has only been tested in bovine, porcine, human, mouse,canine. rat,reactivity reported in scientific literature (PMID: 18700924). Chicken reactivity reported in scientific literature (PMID: 24227655)
Purification Protein G purified
Alternative Name RPE65 (RPE65 Antibody Abstract)
Background Gene Symbol: RPE65
Molecular Weight Theoretical MW: 65 kDa
Gene ID 6121, 19892, 282043
UniProt Q28175
Research Area Signaling, Metabolism, Neurology
Application Notes Western Blot 1:5000-1:10000, Immunohistochemistry 1:250, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:250
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.
Product cited in: Harris, Fisher, Jorgensen, Kaushal, Scott: "CD133 progenitor cells from the bone marrow contribute to retinal pigment epithelium repair." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 2, pp. 457-66, 2009 (PubMed).

Jiang, Ke, Sun, Han, Kaplan, Shao: "Reactivation of uveitogenic T cells by retinal astrocytes derived from experimental autoimmune uveitis-prone B10RIII mice." in: Investigative ophthalmology & visual science, Vol. 49, Issue 1, pp. 282-9, 2008 (PubMed).

Lall, Ferrell, Nagar, Fleisher, McGahan: "Iron regulates L-cystine uptake and glutathione levels in lens epithelial and retinal pigment epithelial cells by its effect on cytosolic aconitase." in: Investigative ophthalmology & visual science, Vol. 49, Issue 1, pp. 310-9, 2008 (PubMed).

Chen, Muckersie, Robertson, Fraczek, Forrester, Xu: "Characterization of a spontaneous mouse retinal pigment epithelial cell line B6-RPE07." in: Investigative ophthalmology & visual science, Vol. 49, Issue 8, pp. 3699-706, 2008 (PubMed).

Jacobson, Aleman, Cideciyan, Heon, Golczak, Beltran, Sumaroka, Schwartz, Roman, Windsor, Wilson, Aguirre, Stone, Palczewski: "Human cone photoreceptor dependence on RPE65 isomerase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 38, pp. 15123-8, 2007 (PubMed).

Jacobson, Yan, Gao, Rincon-Skinner, Rivera, Edwards, Huang, Kaley, Sun: "Aging enhances pressure-induced arterial superoxide formation." in: American journal of physiology. Heart and circulatory physiology, Vol. 293, Issue 3, pp. H1344-50, 2007 (PubMed).

Background publications Thompson, Gyuerues, Fleischer, Bingham, McHenry, Apfelstedt-Sylla, Zrenner, Lorenz, Richards, Jacobson, Sieving, Gal: "Genetics and phenotypes of RPE65 mutations in inherited retinal degeneration." in: Investigative ophthalmology & visual science, Vol. 41, Issue 13, pp. 4293-9, 2000 (PubMed).

Redmond, Yu, Lee, Bok, Hamasaki, Chen, Goletz, Ma, Crouch, Pfeifer: "Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle." in: Nature genetics, Vol. 20, Issue 4, pp. 344-51, 1998 (PubMed).

Nicoletti, Wong, Kawase, Gibson, Yang-Feng, Richards, Thompson: "Molecular characterization of the human gene encoding an abundant 61 kDa protein specific to the retinal pigment epithelium." in: Human molecular genetics, Vol. 4, Issue 4, pp. 641-9, 1995 (PubMed).

Hamel, Tsilou, Pfeffer, Hooks, Detrick, Redmond: "Molecular cloning and expression of RPE65, a novel retinal pigment epithelium-specific microsomal protein that is post-transcriptionally regulated in vitro." in: The Journal of biological chemistry, Vol. 268, Issue 21, pp. 15751-7, 1993 (PubMed).