RPE65 antibody (Retinal Pigment Epithelium-Specific Protein 65kDa) (HRP)

Details for Product anti-RPE65 Antibody No. ABIN314162, Supplier: Log in to see
Antigen
  • LCA2
  • RP20
  • mRPE65
  • rd12
  • sRPE65
  • 65kDa
  • A930029L06Rik
  • Mord1
  • DRPE65
  • RPE65
  • anon-WO0148162.1
  • anon-WO0148163.1
  • beta-diox
  • DmelCG9347
  • CG9347
  • MGC52887
  • MGC154296
  • MGC85437
  • rpe65
  • RPE56c
  • si:ch211-198n5.12
  • retinal pigment epithelium-specific protein 65kDa
  • retinal pigment epithelium 65
  • neither inactivation nor afterpotential B
  • RPE65 homolog
  • retinal pigment epithelium-specific protein 65c
  • RPE65
  • Rpe65
  • ninaB
  • rpe65
  • rpe65c
  • LOC100219959
  • LOC100352270
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
52
50
44
31
20
19
18
18
1
1
1
Host
Mouse
28
26
9
1
Clonality (Clone)
Monoclonal ()
Conjugate
This RPE65 antibody is conjugated to HRP
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
46
24
21
18
17
17
13
13
6
3
1
1
Options
Supplier
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Immunogen Bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]
Clone 401-8B11-3D9
Isotype IgG1 kappa
Specificity This antibody is specific for RPE65.
Purification Protein G purified
Alternative Name RPE65 (RPE65 Antibody Abstract)
Background Gene Symbol: RPE65
Molecular Weight Theoretical MW: 65 kDa
Gene ID 6121
UniProt Q28175
Research Area Signaling, Metabolism, Neurology
Application Notes Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenImmunohistochemistry and western blot. By Western blot, this RPE65 antibody recognizes a band at ~65 kDa, representing bovine RPE65 protein. It has also been tested on recombinant human protein in COS7 cells. It may also be used in immunohistochemistry-Frozen, on murine retinal sections and ICC in porcine. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS
Buffer contains: No Preservative
Preservative Without preservative
Storage 4 °C
Storage Comment Store at 4°C in the dark.
Product cited in: Harris, Fisher, Jorgensen, Kaushal, Scott: "CD133 progenitor cells from the bone marrow contribute to retinal pigment epithelium repair." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 2, pp. 457-66, 2009 (PubMed).

Jiang, Ke, Sun, Han, Kaplan, Shao: "Reactivation of uveitogenic T cells by retinal astrocytes derived from experimental autoimmune uveitis-prone B10RIII mice." in: Investigative ophthalmology & visual science, Vol. 49, Issue 1, pp. 282-9, 2008 (PubMed).

Lall, Ferrell, Nagar, Fleisher, McGahan: "Iron regulates L-cystine uptake and glutathione levels in lens epithelial and retinal pigment epithelial cells by its effect on cytosolic aconitase." in: Investigative ophthalmology & visual science, Vol. 49, Issue 1, pp. 310-9, 2008 (PubMed).

Chen, Muckersie, Robertson, Fraczek, Forrester, Xu: "Characterization of a spontaneous mouse retinal pigment epithelial cell line B6-RPE07." in: Investigative ophthalmology & visual science, Vol. 49, Issue 8, pp. 3699-706, 2008 (PubMed).

Jacobson, Aleman, Cideciyan, Heon, Golczak, Beltran, Sumaroka, Schwartz, Roman, Windsor, Wilson, Aguirre, Stone, Palczewski: "Human cone photoreceptor dependence on RPE65 isomerase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 38, pp. 15123-8, 2007 (PubMed).

Jacobson, Yan, Gao, Rincon-Skinner, Rivera, Edwards, Huang, Kaley, Sun: "Aging enhances pressure-induced arterial superoxide formation." in: American journal of physiology. Heart and circulatory physiology, Vol. 293, Issue 3, pp. H1344-50, 2007 (PubMed).

Background publications Thompson, Gyuerues, Fleischer, Bingham, McHenry, Apfelstedt-Sylla, Zrenner, Lorenz, Richards, Jacobson, Sieving, Gal: "Genetics and phenotypes of RPE65 mutations in inherited retinal degeneration." in: Investigative ophthalmology & visual science, Vol. 41, Issue 13, pp. 4293-9, 2000 (PubMed).

Redmond, Yu, Lee, Bok, Hamasaki, Chen, Goletz, Ma, Crouch, Pfeifer: "Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle." in: Nature genetics, Vol. 20, Issue 4, pp. 344-51, 1998 (PubMed).

Nicoletti, Wong, Kawase, Gibson, Yang-Feng, Richards, Thompson: "Molecular characterization of the human gene encoding an abundant 61 kDa protein specific to the retinal pigment epithelium." in: Human molecular genetics, Vol. 4, Issue 4, pp. 641-9, 1995 (PubMed).

Hamel, Tsilou, Pfeffer, Hooks, Detrick, Redmond: "Molecular cloning and expression of RPE65, a novel retinal pigment epithelium-specific microsomal protein that is post-transcriptionally regulated in vitro." in: The Journal of biological chemistry, Vol. 268, Issue 21, pp. 15751-7, 1993 (PubMed).