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PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody (Biotin)

Details for Product No. ABIN314189, Supplier: Log in to see
Antigen
  • Blimp-1
  • blimp-1
  • blimp1
  • BLIMP1
  • Blimp1
  • CG5249
  • dBlimp-1
  • dBlimp1
  • Dmel\\CG5249
  • GB11750
  • MGC52536
  • nrd
  • prdi-bf1
  • PRDI-BF1
  • prdm1
  • PRDM1
  • ubo
  • wu:fb99c12
  • xblimp1
  • zgc:91885
  • ZNFPR1A1
Epitope
AA 199-409
34
26
15
14
9
8
7
6
5
5
5
5
3
3
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine)
145
52
37
11
7
6
1
1
1
1
Host
Mouse
88
39
29
5
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
9
8
6
5
5
3
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
145
104
21
20
18
8
6
5
3
2
2
2
1
1
1
1
Supplier
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Immunogen A fragment of mouse Blimp-1 corresponding to residues 199-409.
Clone 3H2-E8
Isotype IgG1
Purification Protein G purified
Alternative Name BLIMP1/PRDM1 (PRDM1 Antibody Abstract)
Background Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers inits carboxy terminus. It is a transcriptional repressor that is both required and sufficientto trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1is expressed in, and may be required for terminally differentiated epithelial cells, retinalneurons and other lineage cells. It has also been implicated in the repression ofbeta-interferon (IFN-beta) and c-myc gene expression. Alternate Names: anti-BLIMP 1 antibody, anti-B Lymphocyte Induced Maturation Protein 1 antibody,anti-BLIMP1 antibody, anti-Positive Regulatory Domain I Binding Factor 1 antibody,anti-PR Domain Containing 1 antibody, anti-PRDI BF1 antibody, anti-PRDM1 antibody.
Gene Symbol: PRDM1
Gene ID 639, 12142
UniProt Q60636
Application Notes This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections and Flow Cytometry. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. This antibody does not immunoprecipitate. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.
Recommended dilutions: ELISA 1:100-1:2000, Flow Cytometry 8 µg/mL where cells are used at 2*10^6/mL, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
Supplier Images
Flow Cytometry (FACS) image for anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody (Biotin) (ABIN314189) anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody (Biotin)
Flow Cytometry (FACS) image for anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody (Biotin) (ABIN314189) anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody (Biotin) (Image 2)
Flow Cytometry (FACS) image for anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody (Biotin) (ABIN314189) anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody (Biotin) (Image 3)
Product cited in: John, Clements, Russell, Garrett-Sinha: "Ets-1 regulates plasma cell differentiation by interfering with the activity of the transcription factor Blimp-1." in: The Journal of biological chemistry, Vol. 283, Issue 2, pp. 951-62, 2008

Liu, Leboeuf, Shi, Li, Wang, Shen, Garcia, Shen, Chen, Janin, Chen, Zhao: "Rituximab plus CHOP (R-CHOP) overcomes PRDM1-associated resistance to chemotherapy in patients with diffuse large B-cell lymphoma." in: Blood, Vol. 110, Issue 1, pp. 339-44, 2007

Robertson, Charatsi, Joyner, Koonce, Morgan, Islam, Paterson, Lejsek, Arnold, Kallies, Nutt, Bikoff: "Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice." in: Development (Cambridge, England), Vol. 134, Issue 24, pp. 4335-45, 2007

Severa, Coccia, Fitzgerald: "Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 26188-95, 2006

Gong, Malek: "Cytokine-dependent Blimp-1 expression in activated T cells inhibits IL-2 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 1, pp. 242-52, 2006

Ohinata, Payer, OCarroll, Ancelin, Ono, Sano, Barton, Obukhanych, Nussenzweig, Tarakhovsky, Saitou, Surani: "Blimp1 is a critical determinant of the germ cell lineage in mice." in: Nature, Vol. 436, Issue 7048, pp. 207-13, 2005

Cattoretti, Angelin-Duclos, Shaknovich, Zhou, Wang, Alobeid: "PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage." in: The Journal of pathology, Vol. 206, Issue 1, pp. 76-86, 2005

Lin, Angelin-Duclos, Kuo, Calame: "Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells." in: Molecular and cellular biology, Vol. 22, Issue 13, pp. 4771-80, 2002

Angelin-Duclos, Cattoretti, Lin, Calame: "Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 10, pp. 5462-71, 2000

Background publications Chang, Cattoretti, Calame: "The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development." in: Mechanisms of development, Vol. 117, Issue 1-2, pp. 305-9, 2002

Lin, Wong, Calame: "Repression of c-myc transcription by Blimp-1, an inducer of terminal B cell differentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5312, pp. 596-9, 1997