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Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (FITC)

Details for Product No. ABIN316948, Supplier: Log in to see
Antigen
  • Cd169
  • Siglec-1
  • Sn
  • CD169
  • SIGLEC-1
  • SN
  • dJ1009E24.1
  • SA
  • SIGLEC1
  • p210
  • pSn
Reactivity
Human
86
73
31
13
1
Host
Mouse
75
51
34
7
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
30
17
7
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
119
45
44
28
20
19
19
15
13
10
9
2
2
2
Supplier
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Immunogen Human Rhinovirus (HRV14) treated monocyte-derived dendritic cells.
Clone 30-06-39
Isotype IgG1
Specificity This antibody specifically recognises CD169, otherwise known as Sialoadhesin (Sn). Clone 7-239 completely inhibits erythrocyte rosetting around Sn+ transductants. AM05587LE-N is recommneded for use in Functional Studies.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: Sialoadhesin, Siglec-1, Sialic acid-binding Ig-like lectin 1
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name CD169 / SIGLEC1 (SIGLEC1 Antibody Abstract)
Background CD169 is a type I transmembrane glycoprotein and prototypic member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-1. CD169 is a macrophage-restricted interaction molecule which binds to sialylated ligands on haematopoietic cells, including neutrophils, NK cells, monocytes, a subset of CD8+ T cells and B cells, through recognition of sialic acid in the alpha-2,3- glycosidic linkage. CD169 is highly expressed by dendritic cells and on stromal macrophages in the spleen, bone marrow and lymph nodes and to a lesser extent in liver, lungs and gut, and studies have shown high expression of CD169 on macrophages in the inflammatory conditions rheumatoid arthritis and atherosclerosis.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1
Gene ID 6614
UniProt Q9BZZ2
Application Notes Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Kirchberger, Majdic, Steinberger et al.: "Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 2, pp. 1145-52, 2005 (PubMed).

Hartnell, Steel, Turley et al.: "Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations." in: Blood, Vol. 97, Issue 1, pp. 288-96, 2001 (PubMed).